BMRBj - BMREntryMaster

Found 1 Document (0.523 seconds)

BMRB: 15177

2KJC

Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states

Authors

Arunkumar, A.I., Pennella, M.A., Kong, X., David, G.P.

Assembly

CzrA

Entity

1. CzrA (polymer, Thiol state: not present), 106 monomers, 11972.55 × 2 Da Detail

MAEQYSEINT DTLERVTEIF KALGDYNRIR IMELLSVSEA SVGHISHQLN LSQSNVSHQL KLLKSVHLVK AKRQGQSMIY SLDDIHVATM LKQAIHHANH PKESGL

Total weight

23945.1 Da

Max. entity weight

11972.55 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 54.1 %, Completeness (bb): 79.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.1 % (673 of 1243)	32.7 % (211 of 645)	73.2 % (352 of 481)	94.0 % (110 of 117)
Backbone	79.7 % (505 of 634)	50.2 % (108 of 215)	94.6 % (297 of 314)	95.2 % (100 of 105)
Sidechain	37.4 % (266 of 711)	24.0 % (103 of 430)	56.9 % (153 of 269)	83.3 % (10 of 12)
Aromatic	6.1 % (4 of 66)	6.1 % (2 of 33)	6.1 % (2 of 33)
Methyl	73.1 % (98 of 134)	70.1 % (47 of 67)	76.1 % (51 of 67)

1. CzrA Chain

MAEQYSEINT DTLERVTEIF KALGDYNRIR IMELLSVSEA SVGHISHQLN LSQSNVSHQL KLLKSVHLVK AKRQGQSMIY SLDDIHVATM LKQAIHHANH PKESGL

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	CzrA	[U-100% 13C; U-100% 15N]	1 (±0.1) mM
2	MES	[U-100% 2H]	10 (±0.5) mM
3	sodium chloride	natural abundance	50 (±1.0) mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
4	CzrA	[U-2H; U-15N]; [U-13C]-Ile,Leu,Val-methyl	1 (±0.1) mM
5	MES	[U-100% 2H]	10 (±0.5) mM
6	sodium chloride	natural abundance	50 (±1.0) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 2 Detail

Release date

2007-08-21

Citation

Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states
Arunkumar, A.I., Pennella, M.A., Kong, X., Giedroc, D.P.
Biomol. NMR Assign. (2007), 1, 99-101, PubMed 19636838 , DOI 10.1007/s12104-007-9027-y , Abstract

Related entities 1. CzrA, : 1 : 1 : 5 : 26 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N TROSY