BMRB: 15191

backbone and sidechain order parameters for CaM-nNOSp

Authors

Valentine, K.G., Wand, A.J., Frederick, K.K., Marlow, M.S.

Assembly

CaM-nNOSp

Entity

1. Calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. nNOSp (polymer, Thiol state: not present), 23 monomers, 2578.127 Da Detail

KRRAIGFKKL AEAVKFSAKL MGQ

Total weight

19284.338 Da

Max. entity weight

16706.21 Da

Source organism

Gallus gallus , Homo sapiens

Exptl. method

solution NMR

Data set

order_parameters

Order parameters

194 S² values in 2 lists
Pressure 1 atm, Temperature 308 K, pH 6.5 Detail

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Pressure 1 atm, Temperature 308 K, pH 6.5

Experiment name 2D 1H-15N HSQC, 2D 1H-13C HSQC

List #1 Backbone_order_parameters, Details NMR derived backbone N-H bond vector model-free squared generalized order parameters for Calmodulin in complex with nNOSp determined at 500 and 600 MHz (1H)

List #2 methyl_order_parameters, Details NMR derived side-chain methyl model-free squared generalized order parameters for the symmetry axis of methyl groups of Calmodulin in complex with nNOSp determined with relaxation data obtained at 500 and 600 MHz (1H).

Release date

2015-07-22

Citation

Conformational entropy in molecular recognition by proteins
Frederick, K.K., Marlow, M.S., Valentine, K.G., Wand, A.J.
Nature (2007), 448, 325-329, PubMed 17637663 , DOI 10.1038/nature05959 , Abstract

Related entities 1. Calmodulin, : 11 : 88 entities Detail

Related entities 2. nNOSp, : 1 : 7 : 1 : 11 entities Detail

Interaction partners 2. nNOSp, : 10 interactors Detail

Experiments performed 2 experiments Detail