BMRBj - BMREntryMaster

Found 1 Document (0.793 seconds)

BMRB: 15193

2KV1

1H, 13C, and 15N NMR resonance assignments of Sec95Cys MsrB1 protein

Authors

Sal, L.S., Aachmann, F.L., Kim, H., Gladyshev, V.N., Dikiy, A.

Assembly

MsrB1

Entity

1. MsrB1 (polymer, Thiol state: all free), 124 monomers, 13806.34 Da Detail

MSFCSFFGGE VFQNHFEPGV YVCAKCSYEL FSSHSKYAHS SPWPAFTETI HPDSVTKCPE KNRPEALKVS CGKCGNGLGH EFLNDGPKRG QSRFCIFSSS LKFVPKGKEA AASQGHLEHH HHHH

Formula weight

13806.34 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 82.3 %, Completeness (bb): 94.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.3 % (1178 of 1431)	83.9 % (625 of 745)	78.5 % (441 of 562)	90.3 % (112 of 124)
Backbone	94.5 % (688 of 728)	94.8 % (238 of 251)	94.7 % (342 of 361)	93.1 % (108 of 116)
Sidechain	73.3 % (598 of 816)	78.3 % (387 of 494)	65.9 % (207 of 314)	50.0 % (4 of 8)
Aromatic	12.4 % (24 of 194)	21.6 % (21 of 97)	2.1 % (2 of 96)	100.0 % (1 of 1)
Methyl	94.7 % (72 of 76)	100.0 % (38 of 38)	89.5 % (34 of 38)

1. MsrB1

MSFCSFFGGE VFQNHFEPGV YVCAKCSYEL FSSHSKYAHS SPWPAFTETI HPDSVTKCPE KNRPEALKVS CGKCGNGLGH EFLNDGPKRG QSRFCIFSSS LKFVPKGKEA AASQGHLEHH HHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 5.5 (±0.1), Details U-98% 13C; U-99% 15N

#	Name	Isotope labeling	Concentration
1	MsrB1	[U-98% 13C; U-99% 15N]	1.0 ~ 1.6 mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	DTT	natural abundance	5 (±0.1) mM
4	sodium chloride	natural abundance	10 (±0.1) mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 5.5 (±0.1), Details 13C/15N label

#	Name	Isotope labeling	Concentration
5	MsrB1	[U-98% 13C; U-99% 15N]	1.0 ~ 1.6 mM
6	sodium phosphate	natural abundance	20 (±0.1) mM
7	DTT	natural abundance	5 (±0.1) mM
8	sodium chloride	natural abundance	10 (±0.1) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.4 ppm, Outliers: 3 Detail

Release date

2007-08-21

Citation

NMR assignments of 1H, 13C and 15N spectra of methionine sulfoxide reductase B1 from Mus musculus
Sal, L.S., Aachmann, F.L., Kim, H., Gladyshev, V.N., Dikiy, A.
Biomol. NMR Assign. (2007), 1, 131-133, PubMed 19636847 , DOI 10.1007/s12104-007-9039-7 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9JLC3 released on 2001-01-11
Title MSRB1_MOUSE Entity Methionine-R-sulfoxide reductase B1

Related entities 1. MsrB1, : 1 : 1 : 68 entities Detail

Interaction partners 1. MsrB1, : 4 interactors Detail

More details for 4 interactors identified by 1 citations