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BMRB: 15230

6ZRN

1H, 13C and 15N resonance assignments for the small GTPase RalB in its active conformation

Authors

Prasannan, S., Fenwick, R.BRYN., Campbell, L.J., Evetts, K., Nietlispach, D., Owen, D., Mott, H.R.

Assembly

RalB-GMPPNP

Entity

1. RalB (polymer, Thiol state: not present), 186 monomers, 21106.57 Da Detail

HMAANKSKGQ SSLALHKVIM VGSGGVGKSA LTLQFMYDEF VEDYEPTKAD SYRKKVVLDG EEVQIDILDT AGLEDYAAIR DNYFRSGEGF LLVFSITEHE SFTATAEFRE QILRVKAEED KIPLLVVGNK SDLEERRQVP VEEARSKAEE WGVQYVETSA KTRANVDKVF FDLMREIRTK KMSENK

2. GNP (non-polymer), 522.196 Da
3. MG (non-polymer), 24.305 Da
4. WATER (water), 18.015 × 2 Da

Total weight

21653.07 Da

Max. entity weight

21106.57 Da

Entity Connection

metal coordination 6, hydrogen 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:SER29:OG	3:MG1:MG
2	metal coordination	sing	1:THR47:OG1	3:MG1:MG
3	metal coordination	sing	2:GNP1:O2B	3:MG1:MG
4	metal coordination	sing	2:GNP1:O2G	3:MG1:MG
5	metal coordination	sing	4:HOH1:O	3:MG1:MG
6	metal coordination	sing	4:HOH1:O	3:MG1:MG
7	hydrogen	sing	1:SER29:OG	2:GNP1:O2B
8	hydrogen	sing	1:THR47:OG1	2:GNP1:O2G
9	hydrogen	sing	1:THR47:N	2:GNP1:O2G
10	hydrogen	sing	1:GLY72:N	2:GNP1:O3G

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 92.1 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.1 % (2008 of 2180)	91.5 % (1039 of 1135)	92.7 % (788 of 850)	92.8 % (181 of 195)
Backbone	92.5 % (1027 of 1110)	92.4 % (351 of 380)	92.5 % (506 of 547)	92.9 % (170 of 183)
Sidechain	91.9 % (1144 of 1245)	91.1 % (688 of 755)	93.1 % (445 of 478)	91.7 % (11 of 12)
Aromatic	96.3 % (156 of 162)	96.3 % (78 of 81)	96.2 % (77 of 80)	100.0 % (1 of 1)
Methyl	98.0 % (200 of 204)	98.0 % (100 of 102)	98.0 % (100 of 102)

1. RalB

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Concentration
1	RalB	[U-99% 13C; U-99% 15N]	0.6 mM
2	GMPPNP	none	0.6 mM
3	Magnesium chloride	none	1 mM
4	sodium chloride	none	100 mM
5	D2O	none	10 %
6	sodium azide	none	0.05 %
7	sodium phosphate	none	50 mM

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Concentration
8	RalB	[U-99% 15N]	0.6 mM
9	GMPPNP	none	0.6 mM
10	Magnesium chloride	none	1 mM
11	sodium chloride	none	100 mM
12	D2O	none	10 %
13	sodium azide	none	0.05 %
14	sodium phosphate	none	50 mM

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Concentration
15	RalB	none	0.6 mM
16	GMPPNP	none	0.6 mM
17	Magnesium chloride	none	1 mM
18	sodium chloride	none	100 mM
19	D2O	none	10 %
20	sodium azide	none	0.05 %
21	sodium phosphate	none	50 mM

Heteronucl. T₁

127 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 7.6 Detail

Heteronucl. T₂

127 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 7.6 Detail

Heteronucl. NOE

127 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 7.6 Detail

Heteronucl. T₁/T₂

127 T₁/T₂ values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 7.6 Detail

Order parameters

127 S² values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 7.6 Detail

Release date

2007-04-29

Citation

1H, 13C and 15N resonance assignments for the small G protein RalB in its active conformation
Prasannan, S., Fenwick, R., Campbell, L.J., Evetts, K.A., Nietlispach, D., Owen, D., Mott, H.R.
Biomol. NMR Assign. (2007), 1, 147-149, PubMed 19636851 , DOI 10.1007/s12104-007-9040-1 , Abstract

Related entities 1. RalB, : 1 : 8 : 291 entities Detail

Interaction partners 1. RalB, : 19 interactors Detail

More details for 19 interactors identified by 11 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC