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Found 1 Document (0.697 seconds)

BMRB: 15236

1SOY

1H, 15N backbone chemical shift assignemnt of the G26K,D27S,D31A triple mutant of the protein CyaY

Authors

Pastore, C., Pastore, A., Correia, A.R., Gomes, C.M.

Assembly

CyaYG26KD27SD31K

Entity

1. CyaYG26KD27SD31K (polymer, Thiol state: all free), 108 monomers, 12358.49 Da Detail

GAMNDSEFHR LADQLWLTIE ERLDDWDKSS DIACEINGGV LTITFENGSK IIINRQEPLH QVWLATKQGG YHFDLKGDEW ICDRSGETFW DLLEQAATQQ AGETVSFR

Formula weight

12358.49 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.6 %, Completeness: 29.0 %, Completeness (bb): 61.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	29.0 % (224 of 772)	18.3 % (119 of 649)	85.4 % (105 of 123)
Backbone	61.3 % (203 of 331)	46.0 % (103 of 224)	93.5 % (100 of 107)
Sidechain	4.8 % (21 of 441)	3.8 % (16 of 425)	31.3 % (5 of 16)
Aromatic	14.3 % (10 of 70)	7.7 % (5 of 65)	100.0 % (5 of 5)
Methyl	5.4 % (3 of 56)	5.4 % (3 of 56)

1. CyaYG26KD27SD31K

GAMNDSEFHR LADQLWLTIE ERLDDWDKSS DIACEINGGV LTITFENGSK IIINRQEPLH QVWLATKQGG YHFDLKGDEW ICDRSGETFW DLLEQAATQQ AGETVSFR

Show sample condition

Sample

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CyaYG26KD27SD31K	[U-98% 15N]	0.4 mM
2	NaP		20 mM
3	NaCl		50 mM
4	DTT		2 mM

Release date

2007-05-17

Citation

Dynamics, stability and iron-binding activity of frataxin clinical mutants
Correia, A.R., Pastore, C., Adinolfi, S., Pastore, A., Gomes, C.M.
FEBS J. (2008), 275, 3680-3690, PubMed 18537827 , DOI 10.1111/j.1742-4658.2008.06512.x , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 15234 released on 2008-05-17
    Title 1H, 15N backbone chemical shift assignment of D122Y mutant of the C terminal domain of human frataxin
  BMRB: 15233 released on 2007-05-17
    Title 1H, 15N backbone chemical shift assignment of the G130V mutant of the C terminal domain of human frataxin
  BMRB: 15235 released on 2007-05-17
    Title 1H, 15N backbone chemical shift assignment of the W155R mutant of the C terminal domain of human frataxin

Related entities 1. CyaYG26KD27SD31K, : 1 : 15 : 126 entities Detail

Interaction partners 1. CyaYG26KD27SD31K, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15236_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15236_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	208		91.7 (chain: 1, length: 108)
1	Chemical shift references	chemical_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 108, Sequence coverage: 91.7%
- Total number of assignments
  - ¹H chemical shifts: 104
  - ¹⁵N chemical shifts: 104
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	649	104	16.0
¹⁵N chemical shifts	128	104	81.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	224	99	44.2
¹⁵N chemical shifts	107	99	92.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	425	5	1.2
¹⁵N chemical shifts	21	5	23.8

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Found 1 Document (0.697 seconds)

BMRB: 15236

Sample

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. CyaYG26KD27SD31K, : 1 : 15 : 126 entities Detail

Interaction partners 1. CyaYG26KD27SD31K, : 3 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail