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BMRB: 15249

2KXL

Sequence-Specific 1H, 13C and 15N Resonance Assignments of the Cyclic Nucleotide Binding Domain from a Cyclic Nucleotide-Gated Potassium Channel in Complex with cAMP

Authors

Schunke, S., Stoldt, M., Willbold, D.

Assembly

Cyclic Nucleotide Binding Domain (CNBD) in Complex with cAMP

Entity

1. MlotiCNBDc (polymer, Thiol state: not present), 142 monomers, 14968.09 Da Detail

GSQEVRRGDF VRNWQLVAAV PLFQKLGPAV LVEIVRALRA RTVPAGAVIC RIGEPGDRMF FVVEGSVSVA TPNPVELGPG AFFGEMALIS GEPRSATVSA ATTVSLLSLH SADFQMLCSS SPEIAEIFRK TALERRGAAA SA

2. CMP (non-polymer), 329.206 Da

Total weight

15297.296 Da

Max. entity weight

14968.09 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.5 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.5 % (1493 of 1563)	94.9 % (761 of 802)	95.5 % (593 of 621)	99.3 % (139 of 140)
Backbone	98.4 % (821 of 834)	98.6 % (283 of 287)	98.1 % (406 of 414)	99.2 % (132 of 133)
Sidechain	93.4 % (802 of 859)	92.8 % (478 of 515)	94.1 % (317 of 337)	100.0 % (7 of 7)
Aromatic	68.8 % (66 of 96)	68.8 % (33 of 48)	68.1 % (32 of 47)	100.0 % (1 of 1)
Methyl	100.0 % (188 of 188)	100.0 % (94 of 94)	100.0 % (94 of 94)

1. MlotiCNBDc

GSQEVRRGDF VRNWQLVAAV PLFQKLGPAV LVEIVRALRA RTVPAGAVIC RIGEPGDRMF FVVEGSVSVA TPNPVELGPG AFFGEMALIS GEPRSATVSA ATTVSLLSLH SADFQMLCSS SPEIAEIFRK TALERRGAAA SA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	MlotiCNBDc	[U-13C; U-15N]	0.5 mM
2	potassium phosphate	natural abundance	10 mM
3	potassium chloride	natural abundance	100 mM
4	cAMP	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.02 % v/v
6	D2O	[U-2H]	5 % v/v
7	H2O	natural abundance	95 % v/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Release date

2007-09-16

Citation

Resonance assignment of the cyclic nucleotide binding domain from a cyclic nucleotide-gated K(+) channel in complex with cAMP
Schunke, S., Novak, K., Stoldt, M., Kaupp, U., Willbold, D.
Biomol. NMR Assign. (2007), 1, 179-181, PubMed 19636859 , DOI 10.1007/s12104-007-9049-5 , Abstract

Related entities 1. MlotiCNBDc, : 1 : 1 : 20 : 170 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC