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BMRB: 15263

3LCN

1H, 13C, and 15N Chemical Shift Assignments for NAB2 N-terminal domain

Authors

Grant, R., Marshall, N.J., Yang, J., Fasken, M., Kelly, S., Harreman, M.T., Neuhaus, D., Corbett, A.H., Stewart, M.

Assembly

NAB2 NTD

Entity

1. NAB2 NTD (polymer, Thiol state: not present), 105 monomers, 11455.82 Da Detail

MSQEQYTENL KVIVAEKLAG IPNFNEDIKY VAEYIVLLIV NGGTVESVVD ELASLFDSVS RDTLANVVQT AFFALEALQQ GESAENIVSK IRMMNAQSLG QSDIA

Formula weight

11455.82 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 81.8 %, Completeness (bb): 80.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.8 % (976 of 1193)	84.4 % (515 of 610)	75.1 % (349 of 465)	94.9 % (112 of 118)
Backbone	80.1 % (503 of 628)	87.4 % (187 of 214)	69.0 % (214 of 310)	98.1 % (102 of 104)
Sidechain	85.7 % (570 of 665)	83.1 % (329 of 396)	90.6 % (231 of 255)	71.4 % (10 of 14)
Aromatic	90.6 % (58 of 64)	90.6 % (29 of 32)	90.6 % (29 of 32)
Methyl	94.0 % (141 of 150)	90.7 % (68 of 75)	97.3 % (73 of 75)

1. NAB2 NTD

MSQEQYTENL KVIVAEKLAG IPNFNEDIKY VAEYIVLLIV NGGTVESVVD ELASLFDSVS RDTLANVVQT AFFALEALQQ GESAENIVSK IRMMNAQSLG QSDIA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Release date

2008-02-20

Citation

Structure of the N-terminal Mlp1-binding domain of the Saccharomyces cerevisiae mRNA-binding protein, Nab2
Grant, R.P., Marshall, N.J., Yang, J., Fasken, M.B., Kelly, S.M., Harreman, M.T., Neuhaus, D., Corbett, A.H., Stewart, M.
J. Mol. Biol. (2008), 376, 1048-1059, PubMed 18190927 , DOI 10.1016/j.jmb.2007.11.087 , Abstract

Related entities 1. NAB2 NTD, : 1 : 3 : 1 entities Detail

Interaction partners 1. NAB2 NTD, : 29 interactors Detail

More details for 29 interactors identified by 8 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

3 2D 1H-13C HSQC CT

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

4 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

5 2D 1H-1H NOESY 15N filtered in F2

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

6 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

7 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

8 3D HNHAHB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

9 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

11 3D HCCH-COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

12 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

13 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

14 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NAB2_NTD	[U-98% 13C; U-98% 15N]		2 mM
2	D2O	[U-2H]		10 %
3	H2O	natural abundance		90 %
4	sodium phosphate	natural abundance		25 mM
5	sodium chloride	natural abundance		10 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15263_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15263_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1162		99.0 (chain: 1, length: 105)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 105, Sequence coverage: 99.0%
- Total number of assignments
  - ¹H chemical shifts: 712
  - ¹³C chemical shifts: 339
  - ¹⁵N chemical shifts: 111
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords protein