The NMR solution structure of a pH sensitive,D-amino acid containing conopeptide, mr12 .
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 85.7 % (72 of 84) | 85.7 % (72 of 84) |
Backbone | 96.0 % (24 of 25) | 96.0 % (24 of 25) |
Sidechain | 81.4 % (48 of 59) | 81.4 % (48 of 59) |
Aromatic | 80.0 % (16 of 20) | 80.0 % (16 of 20) |
Methyl | 100.0 % (2 of 2) | 100.0 % (2 of 2) |
1. mr12 at pH5
DWEYHAHPKX NSXWTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mr12 at pH5 | natural abundance | 2.0 mM | |
2 | potassium phosphate | natural abundance | 20 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | mr12 at pH5 | natural abundance | 2.0 mM | |
6 | potassium phosphate | natural abundance | 20 mM | |
7 | D2O | 100 % |