1H, 13C and 15N resonance assignment of 6aJL2(R25G), a highly fibrillogenic lamdaVI light chain variable domain.
NFMLTQPHSV SESPGKTVTI SCTGSSGSIA SNYVQWYQQR PGSSPTTVIY EDNQRPSGVP DRFSGSIDSS SNSASLTISG LKTEDEADYY CQSYDSSNHV VFGGGTKLTV L
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS22:SG | 1:CYS91:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 99.1 % (1186 of 1197) | 99.0 % (608 of 614) | 99.6 % (464 of 466) | 97.4 % (114 of 117) |
Backbone | 99.7 % (652 of 654) | 100.0 % (226 of 226) | 99.7 % (322 of 323) | 99.0 % (104 of 105) |
Sidechain | 98.6 % (635 of 644) | 98.5 % (382 of 388) | 99.6 % (243 of 244) | 83.3 % (10 of 12) |
Aromatic | 98.0 % (96 of 98) | 98.0 % (48 of 49) | 97.9 % (47 of 48) | 100.0 % (1 of 1) |
Methyl | 100.0 % (98 of 98) | 100.0 % (49 of 49) | 100.0 % (49 of 49) |
1. 6aJL2
NFMLTQPHSV SESPGKTVTI SCTGSSGSIA SNYVQWYQQR PGSSPTTVIY EDNQRPSGVP DRFSGSIDSS SNSASLTISG LKTEDEADYY CQSYDSSNHV VFGGGTKLTV LSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 5.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | 6aJL2 | natural abundance | 1.2 mM | |
2 | sodium phosphate | natural abundance | 25 mM | |
3 | sodium azide | natural abundance | 0.05 % | |
4 | H2O | 95 % | ||
5 | D2O | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 5.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | 6aJL2 | [U-95% 15N] | 1.2 mM | |
7 | sodium phosphate | natural abundance | 25 mM | |
8 | sodium azide | natural abundance | 0.05 % | |
9 | H2O | 95 % | ||
10 | D2O | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 5.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | 6aJL2 | [U-95% 13C; U-95% 15N] | 1.2 mM | |
12 | sodium phosphate | natural abundance | 25 mM | |
13 | sodium azide | natural abundance | 0.05 % | |
14 | H2O | 95 % | ||
15 | D2O | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 5.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | 6aJL2 | natural abundance | 1.3 mM | |
17 | sodium phosphate | natural abundance | 25 mM | |
18 | sodium azide | natural abundance | 0.05 % | |
19 | D2O | 100 % |