BMRBj - BMREntryMaster

Found 1 Document (0.527 seconds)

BMRB: 15280

2JZS

1H, 13C and 15N resonance assignment of the oxidized form (Cys67-Cys70) of the N-terminal domain of PilB from Neisseria meningitidis

Authors

Quinternet, M., Beaufils, C., Neiers, F., Tsan, P., Boschi-Muller, S., Averlant-petit, M., Branlant, G., Cung, M.

Assembly

Dsbe ox

Entity

1. Dsbe ox (polymer, Thiol state: all disulfide bound), 144 monomers, 15829.93 Da Detail

MVPHTLSTLK TADNRPASVY LKKDKPTLIK FWASWCPLCL SELGQTEKWA QDAKFSSANL ITVASPGFLH EKKDGDFQKW YAGLNYPKLP VVTDNGGTIA QSLNISVYPS WALIGKDGDV QRIVKGSINE AQALALIRDP NADL

Formula weight

15829.93 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS36:SG	1:CYS39:SG

Source organism

Neisseria meningitidis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 80.7 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.7 % (1359 of 1684)	77.2 % (673 of 872)	83.3 % (549 of 659)	89.5 % (137 of 153)
Backbone	95.6 % (809 of 846)	93.8 % (270 of 288)	96.2 % (407 of 423)	97.8 % (132 of 135)
Sidechain	70.0 % (681 of 973)	69.0 % (403 of 584)	73.6 % (273 of 371)	27.8 % (5 of 18)
Aromatic	11.4 % (16 of 140)	15.7 % (11 of 70)	0.0 % (0 of 65)	100.0 % (5 of 5)
Methyl	93.0 % (160 of 172)	89.5 % (77 of 86)	96.5 % (83 of 86)

1. Dsbe ox

MVPHTLSTLK TADNRPASVY LKKDKPTLIK FWASWCPLCL SELGQTEKWA QDAKFSSANL ITVASPGFLH EKKDGDFQKW YAGLNYPKLP VVTDNGGTIA QSLNISVYPS WALIGKDGDV QRIVKGSINE AQALALIRDP NADL

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	[U-13C; U-15N]		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	internal	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	internal	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	internal	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	internal	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Release date

2007-07-17

Citation

1H, 13C and 15N resonance assignment of the oxidized form (Cys(67)-Cys (70)) of the N-terminal domain of PilB from Neisseria meningitidis
Quinternet, M., Beaufils, C., Neiers, F., Tsan, P., Boschi-Muller, S., Averlant-Petit, M., Branlant, G., Cung, M.
Biomol. NMR Assign. (2007), 1, 143-145, PubMed 19636850 , DOI 10.1007/s12104-007-9038-8 , Abstract

Related entities 1. Dsbe ox, : 1 : 2 : 3 : 50 entities Detail

Experiments performed 11 experiments Detail

1 3D CBCA(CO)NH