1H, 13C and 15N resonance assignment of the oxidized form (Cys67-Cys70) of the N-terminal domain of PilB from Neisseria meningitidis
MVPHTLSTLK TADNRPASVY LKKDKPTLIK FWASWCPLCL SELGQTEKWA QDAKFSSANL ITVASPGFLH EKKDGDFQKW YAGLNYPKLP VVTDNGGTIA QSLNISVYPS WALIGKDGDV QRIVKGSINE AQALALIRDP NADL
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS36:SG | 1:CYS39:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.7 % (1359 of 1684) | 77.2 % (673 of 872) | 83.3 % (549 of 659) | 89.5 % (137 of 153) |
Backbone | 95.6 % (809 of 846) | 93.8 % (270 of 288) | 96.2 % (407 of 423) | 97.8 % (132 of 135) |
Sidechain | 70.0 % (681 of 973) | 69.0 % (403 of 584) | 73.6 % (273 of 371) | 27.8 % (5 of 18) |
Aromatic | 11.4 % (16 of 140) | 15.7 % (11 of 70) | 0.0 % (0 of 65) | 100.0 % (5 of 5) |
Methyl | 93.0 % (160 of 172) | 89.5 % (77 of 86) | 96.5 % (83 of 86) |
1. Dsbe ox
MVPHTLSTLK TADNRPASVY LKKDKPTLIK FWASWCPLCL SELGQTEKWA QDAKFSSANL ITVASPGFLH EKKDGDFQKW YAGLNYPKLP VVTDNGGTIA QSLNISVYPS WALIGKDGDV QRIVKGSINE AQALALIRDP NADLSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | potassium phosphate | [U-13C; U-15N] | 50 mM |