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BMRB: 15333

2JRJ

Resonance Assignment of the human Pirh2 RING domain; Northeast Structural Genomics Consortium Target HT2B

Authors

Sheng, Y., Lemak, A., Laister, R., Wu, B., Yee, A., Arrowsmith, C.H.

Assembly

Pirh2 RING domain

Entity

1. Pirh2 RING domain (polymer, Thiol state: all other bound), 52 monomers, 5994.903 Da Detail

ENVSQQNCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCM HS

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

6125.7207 Da

Max. entity weight

5994.903 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.9 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.9 % (552 of 594)	92.9 % (287 of 309)	92.7 % (215 of 232)	94.3 % (50 of 53)
Backbone	98.0 % (300 of 306)	98.1 % (101 of 103)	97.4 % (150 of 154)	100.0 % (49 of 49)
Sidechain	89.3 % (302 of 338)	90.3 % (186 of 206)	89.8 % (115 of 128)	25.0 % (1 of 4)
Aromatic	61.1 % (22 of 36)	72.2 % (13 of 18)	50.0 % (9 of 18)
Methyl	92.6 % (50 of 54)	92.6 % (25 of 27)	92.6 % (25 of 27)

1. Pirh2 RING domain

ENVSQQNCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCM HS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Pirh2 RING domain	[U-13C; U-15N]	0.7 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	150 mM
4	ZnCl2	natural abundance	10 uM
5	DTT	[U-99% 2H]	2 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	Pirh2 RING domain	[U-13C; U-15N]	0.7 mM
9	sodium phosphate	natural abundance	25 mM
10	potassium chloride	natural abundance	150 mM
11	ZnCl2	natural abundance	10 uM
12	DTT	[U-99% 2H]	2 mM
13	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.5 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.5 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

Release date

2007-07-29

Citation

Molecular basis of Pirh2-mediated p53 ubiquitylation
Sheng, Y., Laister, R.C., Lemak, A., Wu, B., Tai, E., Duran, S., Lukin, J., Sunnerhagen, M., Srisailam, S., Karra, M., Benchimol, S., Arrowsmith, C.H.
Nat. Struct. Mol. Biol. (2008), 15, 1334-1342, PubMed 19043414 , DOI 10.1038/nsmb.1521 , Abstract

Related entities 1. Pirh2 RING domain, : 1 : 5 : 262 entities Detail

Interaction partners 1. Pirh2 RING domain, : 32 interactors Detail

More details for 32 interactors identified by 9 citations

Experiments performed 8 experiments Detail

1 3D C(CO)NH