BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	88.8 % (1266 of 1426)	93.0 % (700 of 753)	81.0 % (442 of 546)	97.6 % (124 of 127)
Backbone	96.7 % (669 of 692)	96.6 % (227 of 235)	96.5 % (333 of 345)	97.3 % (109 of 112)
Sidechain	83.4 % (705 of 845)	91.3 % (473 of 518)	69.6 % (217 of 312)	100.0 % (15 of 15)
Aromatic	71.4 % (80 of 112)	89.3 % (50 of 56)	51.9 % (28 of 54)	100.0 % (2 of 2)
Methyl	83.3 % (100 of 120)	96.7 % (58 of 60)	70.0 % (42 of 60)

1. JARID1C

SMNELEAQTR VKLNYLDQIA KFWEIQGSSL KIPNVERRIL DLYSLSKIVV EEGGYEAICK DRRWARVAQR LNYPPGKNIG SLLRSHYERI VYPYEMYQSG ANLVCNTRPF DNEEKDK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 13C,15N double -labelled Bright/ARID domain in 100%D2O

#	Name	Isotope labeling	Concentration
6	JARID1C	[U-13C; U-15N]	1 mM
7	sodium phosphate	natural abundance	50 mM
8	sodium chloride	natural abundance	50 mM
9	sodium azide	natural abundance	0.02 %
10	DTT	natural abundance	5 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N labelled Bright/ARID domain in 100%D2O

#	Name	Isotope labeling	Concentration
11	JARID1C	[U-100% 15N]	1 mM
12	sodium phosphate	natural abundance	50 mM
13	sodium chloride	natural abundance	50 mM
14	sodium azide	natural abundance	0.02 %
15	DTT	natural abundance	5 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 13C,15N double -labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
16	JARID1C	[U-13C; U-15N]	1 mM
17	sodium phosphate	natural abundance	50 mM
18	sodium chloride	natural abundance	50 mM
19	sodium azide	natural abundance	0.02 %
20	DTT	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 1.93 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JRZ, Strand ID: A Detail

Release date

2007-12-09

Citation

Backbone and sidechain 1H, 13C and 15N resonance assignments of the Bright/ARID domain from the human JARID1C (SMCX) protein
Koehler, C., Bishop, S., Dowler, E.F., Schmieder, P., Diehl, A., Oschkinat, H., Ball, L.J.
Biomol. NMR Assign. (2008), 2, 9-11, PubMed 19636912 , DOI 10.1007/s12104-007-9071-7 , Abstract

Related entities 1. JARID1C, : 1 : 49 entities Detail

Interaction partners 1. JARID1C, : 9 interactors Detail

More details for 9 interactors identified by 6 citations

Experiments performed 13 experiments Detail

1 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

2 3D CBCANH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

3 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

4 2D 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

5 3D HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

6 3D HNCACO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

7 3D HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

8 3D H(CCCO)NH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

9 (H)CC(CO)NH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

10 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 13C,15N double -labelled Bright/ARID domain in 100%D2O

#	Name	Isotope labeling	Concentration
6	JARID1C	[U-13C; U-15N]	1 mM
7	sodium phosphate	natural abundance	50 mM
8	sodium chloride	natural abundance	50 mM
9	sodium azide	natural abundance	0.02 %
10	DTT	natural abundance	5 mM

11 3D HCCH-COSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 13C,15N double -labelled Bright/ARID domain in 100%D2O

#	Name	Isotope labeling	Concentration
6	JARID1C	[U-13C; U-15N]	1 mM
7	sodium phosphate	natural abundance	50 mM
8	sodium chloride	natural abundance	50 mM
9	sodium azide	natural abundance	0.02 %
10	DTT	natural abundance	5 mM

12 3D 1H-15N NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

13 3D 1H-13C NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 6.0, Details Uniformly 15N-labelled Bright/ARID domain in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	JARID1C	[U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.02 %
5	DTT	natural abundance	5 mM

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_15348_2jrz.nef
Input source #2: Coordindates	2jrz.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
SMNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERRILDLYSLSKIVVEEGGYEAICKDRRWARVAQRLNYPPGKNIGSLLRSHYERIVYPYEMYQSG
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SMNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERRILDLYSLSKIVVEEGGYEAICKDRRWARVAQRLNYPPGKNIGSLLRSHYERIVYPYEMYQSG

-------110-------
ANLVCNTRPFDNEEKDK
|||||||||||||||||
ANLVCNTRPFDNEEKDK

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	117	0	0	100.0

Content subtype: combined_15348_2jrz.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1260		100.0 (chain: A, length: 117)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	957 (1)	noe	97.4 (chain: A, length: 117)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	78 (1)	hbond	58.1 (chain: A, length: 117)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	209 (209)	.	98.3 (chain: A, length: 117)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 704
  - ¹³C chemical shifts: 432
  - ¹⁵N chemical shifts: 124
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	753	704	93.5
¹³C chemical shifts	546	432	79.1
¹⁵N chemical shifts	137	124	90.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	235	230	97.9
¹³C chemical shifts	234	225	96.2
¹⁵N chemical shifts	112	109	97.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	518	474	91.5
¹³C chemical shifts	312	207	66.3
¹⁵N chemical shifts	25	15	60.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	62	62	100.0
¹³C chemical shifts	62	38	61.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	56	50	89.3
¹³C chemical shifts	54	28	51.9
¹⁵N chemical shifts	2	2	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 97.4%
- Total number of restraints: 949
- Weight of restraints: 1.0 (949)
- Potential type of restraints: square-well-parabolic (949)
- Range of distance target values: 2.65, 4.4 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 58.1%
  - Total number of restraints: 78
  - Weight of restraints: 1.0 (78)
  - Potential type of restraints: square-well-parabolic (78)
  - Range of distance target values: 1.8, 2.6 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 117, Sequence coverage: 98.3%
- Total number of restraints: 209
- Total number of restraints per polymer type: 209
- Weight of restraints: 1.0 (209)
- Potential type of restraints: square-well-parabolic (209)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords Bright/ARID domain, helical, JARID1C

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Found 1 Document (0.868 seconds)

BMRB: 15348

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. JARID1C, : 1 : 49 entities Detail

Interaction partners 1. JARID1C, : 9 interactors Detail

Experiments performed 13 experiments Detail

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NMR combined restraints 5 contents Detail