BMRBj - BMREntryMaster

Found 1 Document (0.247 seconds)

BMRB: 15391

Tetrameric structure of KIA7 peptide

Authors

Lopez de la Osa, J., Gonzalez, C., Laurents, D.V., Chakrabartty, A., Bateman, D.A.

Assembly

KIA7 peptide tetramer

Entity

1. KIA7 peptide tetramer (polymer, Thiol state: not present), 22 monomers, 1867.261 × 4 Da Detail

XAKAAAAAIK AIAAIIKAGG YX

Total weight

7469.044 Da

Max. entity weight

1867.261 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 100.0 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	100.0 % (102 of 102)	100.0 % (102 of 102)
Backbone	100.0 % (42 of 42)	100.0 % (42 of 42)
Sidechain	100.0 % (60 of 60)	100.0 % (60 of 60)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (18 of 18)	100.0 % (18 of 18)

1. KIA7

XAKAAAAAIK AIAAIIKAGG YX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278.15 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	sodium acetate	natural abundance	10 mM
2	sodium chloride	natural abundance	200 mM
3	DSS	natural abundance	0.005 mM
4	H2O		90 %
5	D2O		10 %

Release date

2008-03-12

Citation

Getting specificity from simplicity in putative proteins from the prebiotic earth
Lopez de la Osa, J., Bateman, D.A., Ho, S., Gonzalez, C., Chakrabartty, A., Laurents, D.V.
Proc. Natl. Acad. Sci. U. S. A. (2007), 104, 14941-14946, PubMed 17855563 , DOI 10.1073/pnas.0706876104 , Abstract

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	KIA7BRMBlist_1	Warning	145		90.9 (chain: 1, length: 22)
1	Chemical shift references	KIA7BRMBlist	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
10	LYS	HZ1	7.65
10	LYS	HZ2	7.65
10	LYS	HZ3	7.65
17	LYS	HZ1	7.59
17	LYS	HZ2	7.59
17	LYS	HZ3	7.59
21	TYR	HH	9.32

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	106	102	96.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	42	97.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	63	60	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	18	94.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	4	100.0

Keywords oligomer, peptide, prebiotic

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15391_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.247 seconds)

BMRB: 15391

Sample

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail