BMRBj - BMREntryMaster

Found 1 Document (1.36 seconds)

BMRB: 15397

NH, NN, C, CA and CB chemical shift assignment of the transcriptional activation domain 1 of the nuclear hormone receptor coactivator ACTR in the free state

Authors

Ebert, M., Bae, S., Dyson, H.JANE., Wright, P.E.

Assembly

ACTR

Entity

1. ACTR (polymer, Thiol state: not present), 71 monomers, 7701.357 Da Detail

GTQNRPLLRN SLDDLVGPPS NLEGQSDERA LLDQLHTLLS NTDATGLEEI DRALGIPELV NQGQALEPKQ D

Formula weight

7701.357 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 43.0 %, Completeness (bb): 76.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.0 % (339 of 788)	20.8 % (86 of 413)	63.8 % (190 of 298)	81.8 % (63 of 77)
Backbone	76.0 % (316 of 416)	48.3 % (69 of 143)	88.9 % (184 of 207)	95.5 % (63 of 66)
Sidechain	18.8 % (82 of 437)	6.3 % (17 of 270)	41.7 % (65 of 156)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 4)	0.0 % (0 of 2)	0.0 % (0 of 2)
Methyl	14.8 % (13 of 88)	11.4 % (5 of 44)	18.2 % (8 of 44)

1. ACTR

GTQNRPLLRN SLDDLVGPPS NLEGQSDERA LLDQLHTLLS NTDATGLEEI DRALGIPELV NQGQALEPKQ D

Show sample condition

Sample

Pressure 1 atm, Temperature 304.15 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	ACTR	[U-98% 13C; U-98% 15N]		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.715615 ppm	internal	indirect	0.2514495
¹H	water	protons	4.715615 ppm	internal	direct	1.0
¹⁵N	water	protons	4.715615 ppm	internal	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.715615 ppm	internal	indirect	0.2514495
¹H	water	protons	4.715615 ppm	internal	direct	1.0
¹⁵N	water	protons	4.715615 ppm	internal	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.715615 ppm	internal	indirect	0.2514495
¹H	water	protons	4.715615 ppm	internal	direct	1.0
¹⁵N	water	protons	4.715615 ppm	internal	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 2 Detail

Release date

2008-04-03

Citation

NMR relaxation study of the complex formed between CBP and the activation domain of the nuclear hormone receptor coactivator ACTR
Ebert, M., Bae, S., Dyson, H.JANE., Wright, P.E.
Biochemistry (2008), 47, 1299-1308, PubMed 18177052 , DOI 10.1021/bi701767j , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 15398 released on 2008-04-03
Title NH, NN, C and CA chemical shift assignment of the nuclear coactivator binding domain of CBP in the free state

Related entities 1. ACTR, : 2 : 25 entities Detail

Interaction partners 1. ACTR, : 57 interactors Detail

More details for 57 interactors identified by 28 citations

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15397_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15397_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	NH_NN_C_CA_and_CB_shifts	Warning	306		94.4 (chain: 1, length: 71)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: NH_NN_C_CA_and_CB_shifts
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 71, Sequence coverage: 94.4%
- Total number of assignments
  - ¹³C chemical shifts: 182
  - ¹H chemical shifts: 62
  - ¹⁵N chemical shifts: 62
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	298	182	61.1
¹H chemical shifts	413	62	15.0
¹⁵N chemical shifts	81	62	76.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	142	124	87.3
¹H chemical shifts	143	62	43.4
¹⁵N chemical shifts	66	62	93.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	156	58	37.2
¹H chemical shifts	270	0	0.0
¹⁵N chemical shifts	15	0	0.0

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Found 1 Document (1.36 seconds)

BMRB: 15397

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. ACTR, : 2 : 25 entities Detail

Interaction partners 1. ACTR, : 57 interactors Detail

Experiments performed 5 experiments Detail

Instrument

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Instrument

Sample

Instrument

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Chemical shift validation 3 contents Detail