Backbone and side chain chemical shift assignments of the F153-to-5-flurotryptophan mutant of human cardiac troponin C
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEXLEFMKGV E
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.1 % (1418 of 1838) | 78.1 % (750 of 960) | 72.7 % (516 of 710) | 90.5 % (152 of 168) |
Backbone | 82.6 % (790 of 956) | 95.8 % (316 of 330) | 69.2 % (324 of 468) | 94.9 % (150 of 158) |
Sidechain | 74.9 % (771 of 1030) | 68.9 % (434 of 630) | 85.9 % (335 of 390) | 20.0 % (2 of 10) |
Aromatic | 24.0 % (25 of 104) | 25.0 % (13 of 52) | 23.1 % (12 of 52) | |
Methyl | 100.0 % (148 of 148) | 100.0 % (74 of 74) | 100.0 % (74 of 74) |
1. F153(FTR)
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEXLEFMKGV ESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.5) K, pH 6.7 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | F153(FTR) | natural abundance | 1.0 (±0.2) mM | |
2 | Calcium | natural abundance | 10 (±1.0) mM | |
3 | potassium chloride | natural abundance | 100 (±10.0) mM | |
4 | H2O | 90 mM | ||
5 | D2O | 10 mM |