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BMRB: 15402

3URG

Calcium binding protein in the calcium-binding form

Authors

Zhang, X., Hu, Y., Jin, C.

Assembly

ccbp

Entity

1. Alr1010 protein (polymer, Thiol state: all free), 126 monomers, 14750.25 Da Detail

MASVERDETR EHRIETEIIV DAEDKEERAM GWYYYLDDTL EFPFMGKWKK KSRKTSTIEE KTVEVLGMAP DDECLKDMYV EVADIGGKDD DVYTAKLSDI EAIDVDDDTQ EAIADWLYWL ARGYKF

2. CA (non-polymer), 40.078 Da

Total weight

14790.328 Da

Max. entity weight

14750.25 Da

Source organism

Anabaena sp.

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.4 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.4 % (1333 of 1474)	87.3 % (666 of 763)	92.4 % (538 of 582)	100.0 % (129 of 129)
Backbone	98.1 % (738 of 752)	96.1 % (246 of 256)	98.9 % (368 of 372)	100.0 % (124 of 124)
Sidechain	85.2 % (717 of 842)	83.0 % (421 of 507)	88.2 % (291 of 330)	100.0 % (5 of 5)
Aromatic	49.3 % (68 of 138)	53.6 % (37 of 69)	41.5 % (27 of 65)	100.0 % (4 of 4)
Methyl	98.4 % (126 of 128)	96.9 % (62 of 64)	100.0 % (64 of 64)

1. Alr1010 protein

MASVERDETR EHRIETEIIV DAEDKEERAM GWYYYLDDTL EFPFMGKWKK KSRKTSTIEE KTVEVLGMAP DDECLKDMYV EVADIGGKDD DVYTAKLSDI EAIDVDDDTQ EAIADWLYWL ARGYKF

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5, Details 1mM Ccbp U-15N,13C, 20mM Tris, 220mM NaCl,10mM DTT; 90% H2O, 10% D2O

#	Name	Isotope labeling	Concentration
1	Alr1010 protein	[U-95% 13C; U-95% 15N]	1 mM
2	Tris		20 mM
3	NaCl		220 mM
4	DTT		10 mM
5	H2O		90 %
6	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.101329

Referencing offset: -0.05 ppm, Outliers: 4 Detail

Release date

2011-05-02

Citation

Structures of Anabaena calcium-binding protein CcbP: insights into Ca2+ signaling during heterocyst differentiation
Hu, Y., Zhang, X., Shi, Y., Zhou, Y., Zhang, W., Su, X., Xia, B., Zhao, J., Jin, C.
J. Biol. Chem. (2011), 286, 12381-12388, PubMed 21330362 , DOI 10.1074/jbc.M110.201186 , Abstract

Related entities 1. Alr1010 protein, : 1 : 3 : 2 entities Detail

Experiments performed 6 experiments Detail

1 3D_13C-separated_NOESY