NMR Assignment of Cdc42(T35A), and Active Switch I mutant of Cdc42
MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPAVFDNY AVTVMIGGEP YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.2 % (1562 of 2077) | 67.8 % (727 of 1072) | 80.0 % (662 of 827) | 97.2 % (173 of 178) |
Backbone | 96.4 % (1006 of 1044) | 96.6 % (341 of 353) | 95.6 % (502 of 525) | 98.2 % (163 of 166) |
Sidechain | 59.7 % (718 of 1202) | 53.7 % (386 of 719) | 68.4 % (322 of 471) | 83.3 % (10 of 12) |
Aromatic | 7.1 % (11 of 156) | 12.8 % (10 of 78) | 0.0 % (0 of 77) | 100.0 % (1 of 1) |
Methyl | 71.2 % (161 of 226) | 73.5 % (83 of 113) | 69.0 % (78 of 113) |
1. Cdc42(T35A)
MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPAVFDNY AVTVMIGGEP YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALEPressure 1 atm, Temperature 298 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cdc42(T35A) | [U-100% 13C; U-100% 15N] | 0.3 ~ 0.5 mM |
Varian INOVA - 500 MHz
State isotropic, Pressure 1 atm, Temperature 298 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cdc42(T35A) | [U-100% 13C; U-100% 15N] | 0.3 ~ 0.5 mM |
Properties
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneContent subtype: bmr15424_3.str
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPAVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPE ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPAVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRL.PLSYPQTDVFLVCFSVVSPSSFENVKEKWVPE -------110-------120-------130-------140-------150-------160-------170-------- ITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALE |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALE
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 1072 | 700 | 65.3 |
13C chemical shifts | 827 | 643 | 77.8 |
15N chemical shifts | 182 | 172 | 94.5 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 353 | 342 | 96.9 |
13C chemical shifts | 356 | 336 | 94.4 |
15N chemical shifts | 166 | 163 | 98.2 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 719 | 358 | 49.8 |
13C chemical shifts | 471 | 307 | 65.2 |
15N chemical shifts | 16 | 9 | 56.2 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 115 | 76 | 66.1 |
13C chemical shifts | 115 | 70 | 60.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 78 | 10 | 12.8 |
13C chemical shifts | 77 | 0 | 0.0 |
15N chemical shifts | 1 | 1 | 100.0 |