BMRBj - BMREntryMaster

Found 1 Document (0.475 seconds)

BMRB: 15436

2JUQ

alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR

Authors

Feng, Z., Ellison, M.

Assembly

alpha-RgIA

Entity

1. alpha-RgIA (polymer, Thiol state: all disulfide bound), 13 monomers, 1576.851 Da Detail

GCCSDVRCRY RCR

Formula weight

1576.851 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS8:SG
2	disulfide	sing	1:CYS3:SG	1:CYS12:SG

Source organism

Conus regius

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.4 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	97.4 % (74 of 76)	97.4 % (74 of 76)
Backbone	96.3 % (26 of 27)	96.3 % (26 of 27)
Sidechain	98.0 % (48 of 49)	98.0 % (48 of 49)
Aromatic	75.0 % (3 of 4)	75.0 % (3 of 4)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. alpha-RgIA

GCCSDVRCRY RCR

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Temperature 298 K, pH 4.6

#	Name	Isotope labeling	Concentration
1	alpha-RgIA	natural abundance	1.0 (±0.2) mM
2	H2O		95 %
3	D2O		5 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2JUQ, Strand ID: A Detail

Release date

2008-03-12

Citation

Alpha-RgIA, a novel conotoxin that blocks the alpha9alpha10 nAChR: structure and identification of key receptor-binding residues
Ellison, M., Feng, Z., Park, A.J., Zhang, X., Olivera, B.M., McIntosh, M.J., Norton, R.S.
J. Mol. Biol. (2008), 377, 1216-1227, PubMed 18295795 , DOI 10.1016/j.jmb.2008.01.082 , Abstract

Related entities 1. alpha-RgIA, : 1 : 5 : 11 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:2:CYS:SG	1:8:CYS:SG	unknown	unknown	n/a
1:3:CYS:SG	1:12:CYS:SG	unknown	unknown	n/a

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	78		100.0 (chain: 1, length: 13)
1	Chemical shift references	chemical_shift_reference_1	OK	1		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	76	74	97.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	26	96.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	49	48	98.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	2	2	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	3	75.0

Keywords alpha-conotoxin, nicotinic acetylcholine receptor, NMR

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15436_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

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Found 1 Document (0.475 seconds)

BMRB: 15436

Sample

Related entities 1. alpha-RgIA, : 1 : 5 : 11 entities Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail