Assignment, structure, and dynamics of de novo designed protein S836
MYGKLNDLLE DLQEVLKHVN QHWQGGQKNM NKVDHHLQNV IEDIHDFMQG GGSGGKLQEM MKEFQQVLDE IKQQLQGGDN SLHNVHENIK EIFHHLEELV HR
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.2 % (1155 of 1239) | 93.7 % (612 of 653) | 90.9 % (421 of 463) | 99.2 % (122 of 123) |
Backbone | 98.7 % (604 of 612) | 96.7 % (207 of 214) | 100.0 % (296 of 296) | 99.0 % (101 of 102) |
Sidechain | 89.4 % (643 of 719) | 92.3 % (405 of 439) | 83.8 % (217 of 259) | 100.0 % (21 of 21) |
Aromatic | 38.9 % (35 of 90) | 60.0 % (27 of 45) | 15.9 % (7 of 44) | 100.0 % (1 of 1) |
Methyl | 96.9 % (93 of 96) | 93.8 % (45 of 48) | 100.0 % (48 of 48) |
1. S836
MYGKLNDLLE DLQEVLKHVN QHWQGGQKNM NKVDHHLQNV IEDIHDFMQG GGSGGKLQEM MKEFQQVLDE IKQQLQGGDN SLHNVHENIK EIFHHLEELV HRPressure 1 atm, Temperature 298 K, pH 4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | S836 | [U-15N] | 1.0 ~ 2.0 mM | |
2 | D2O | [U-2H] | 10 % | |
3 | acetic acid - sodium acetate | natural abundance | 50 mM |
Pressure 1 atm, Temperature 298 K, pH 4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | S836 | [U-13C; U-15N] | 2 mM | |
5 | D2O | [U-2H] | 10 % | |
6 | acetic acid - sodium acetate | [U-13C] | 50 mM |