BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	93.6 % (973 of 1039)	91.5 % (495 of 541)	95.3 % (382 of 401)	99.0 % (96 of 97)
Backbone	97.5 % (513 of 526)	97.8 % (174 of 178)	97.0 % (255 of 263)	98.8 % (84 of 85)
Sidechain	91.0 % (544 of 598)	88.4 % (321 of 363)	94.6 % (211 of 223)	100.0 % (12 of 12)
Aromatic	91.2 % (31 of 34)	88.2 % (15 of 17)	93.8 % (15 of 16)	100.0 % (1 of 1)
Methyl	96.7 % (116 of 120)	95.0 % (57 of 60)	98.3 % (59 of 60)

1. OST (On-SighT)

MAECVSQAID INEPIGNLKK LLEPRLQCSL DAHEICLQDI QLDPDRSLFD QGVKTDGTVQ LSVQVISYQG MEPKLNILEI VKTAETVEW

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM
7	GABPa OST	[U-100% 15N]	1 mM
8	DTT	natural abundance	2 mM

Sample #3

Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details 10% 13C- and uniformly 15N- labeled GABPa OST

#	Name	Isotope labeling	Concentration
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	50 mM
11	GABPa OST	[U-10% 13C; U-100% 15N]	1 mM
12	DTT	natural abundance	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	direct	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	direct	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	direct	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	direct	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.37 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JUO, Strand ID: A Detail

Heteronucl. T₁

78 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 500 MHz, Temperature 298.0 K, pH 7.0 Detail

Heteronucl. T₂

78 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, Temperature 298.0 K, pH 7.0 Detail

Heteronucl. NOE

77 NOE values in 1 lists
Value type peak height, Field strength (¹H) 500 MHz, Temperature 298.0 K, pH 7.0 Detail

Heteronucl. T₁/T₂

78 T₁/T₂ values in 1 lists
Field strength (¹H) 500 MHz, Temperature 298.0 K, pH 7.0 Detail

Order parameters

77 S² values in 1 lists
Temperature 298.0 K, pH 7.0 Detail

Coupling constant

57 J values in 1 lists
Field strength (¹H) 600 MHz, Temperature 298.0 K, pH 7.0 Detail

RDC

50 RDC values in 1 lists
Field strength (¹H) 600 MHz, Temperature 298.0 K, pH 7.0 Detail

Release date

2008-07-27

Citation

Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABP alpha
Kang, H., Nelson, M.L., Mackereth, C.D., Schaerpf, M., Graves, B.J., McIntosh, L.P.
J. Mol. Biol. (2008), 377, 636-646, PubMed 18295234 , DOI 10.1016/j.jmb.2008.01.054 , Abstract

Related entities 1. OST domain in GABP alpha, : 1 : 2 : 12 entities Detail

Interaction partners 1. OST domain in GABP alpha, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM
7	GABPa OST	[U-100% 15N]	1 mM
8	DTT	natural abundance	2 mM

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details 10% 13C- and uniformly 15N- labeled GABPa OST

#	Name	Isotope labeling	Concentration
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	50 mM
11	GABPa OST	[U-10% 13C; U-100% 15N]	1 mM
12	DTT	natural abundance	2 mM

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

4 3D HNCO

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

5 3D HNCA

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

6 3D HNCACB

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

7 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM
7	GABPa OST	[U-100% 15N]	1 mM
8	DTT	natural abundance	2 mM

8 3D 1H-15N TOCSY

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

9 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

10 simultaneous CN NOESY

Instrument

Varian INOVA - 800 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

11 EZ-HMQC-NH2

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

12 N-CG spin echo

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

13 CO-CG spin echo

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 13C/15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	20 mM
3	GABPa OST	[U-100% 13C; U-100% 15N]	1 mM
4	DTT	natural abundance	2 mM

14 15N IPAP

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM
7	GABPa OST	[U-100% 15N]	1 mM
8	DTT	natural abundance	2 mM

15 3D HNHB

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM
7	GABPa OST	[U-100% 15N]	1 mM
8	DTT	natural abundance	2 mM

16 T1 T2 HNNOE

Instrument

Varian Unity - 500 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM
7	GABPa OST	[U-100% 15N]	1 mM
8	DTT	natural abundance	2 mM

17 3D HNHA

Instrument

Varian INOVA - 600 MHz triple resonance gradient probes

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298.0 K, pH 7.0, Details uniformly 15N labeled GABPa OST

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM
7	GABPa OST	[U-100% 15N]	1 mM
8	DTT	natural abundance	2 mM

nullKeywords Ets transcription factor, GABP, NMR ENSEMBLE, OST, GA-binding protein, NMR ensemble, transcription factor, ubiquitin

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Found 1 Document (1.429 seconds)

BMRB: 15451

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. OST domain in GABP alpha, : 1 : 2 : 12 entities Detail

Interaction partners 1. OST domain in GABP alpha, : 2 interactors Detail

Experiments performed 17 experiments Detail

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