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BMRB: 15469

2JZF

NMR assignment of the domain 513-651 from the SARS-CoV nonstructural protein nsp3

Authors

Chatterjee, A., Johnson, M.A., Serrano, P., Pedrini, B., Joseph, J., Saikatendu, K., Neuman, B.W., Wilson, I.A., Stevens, R., Buchmeier, M.J., Kuhn, P., Wuthrich, K.

Assembly

nsp3(513-651)

Entity

1. nsp3(513-651) (polymer, Thiol state: all free), 143 monomers, 15841.21 Da Detail

GSHMVLPSEA PNAKEEILGT VSWNLREMLA HAEETRKLMP ICMDVRAIMA TIQRKYKGIK IQEGIVDYGV RFFFYTSKEP VASIITKLNS LNEPLVTMPI GYVTHGFNLE EAARCMRSLK APAVVSVSSP DAVTTYNGYL TSS

Formula weight

15841.21 Da

Source organism

Severe acute respiratory syndrome-related coronavirus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 93.7 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.7 % (1547 of 1651)	92.4 % (788 of 853)	94.3 % (617 of 654)	98.6 % (142 of 144)
Backbone	98.0 % (825 of 842)	98.3 % (281 of 286)	97.6 % (411 of 421)	98.5 % (133 of 135)
Sidechain	90.7 % (856 of 944)	89.4 % (507 of 567)	92.4 % (340 of 368)	100.0 % (9 of 9)
Aromatic	57.1 % (64 of 112)	58.9 % (33 of 56)	54.5 % (30 of 55)	100.0 % (1 of 1)
Methyl	98.3 % (173 of 176)	98.9 % (87 of 88)	97.7 % (86 of 88)

1. nsp3(513-651)

GSHMVLPSEA PNAKEEILGT VSWNLREMLA HAEETRKLMP ICMDVRAIMA TIQRKYKGIK IQEGIVDYGV RFFFYTSKEP VASIITKLNS LNEPLVTMPI GYVTHGFNLE EAARCMRSLK APAVVSVSSP DAVTTYNGYL TSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	nsp3(513-651)	[U-99% 13C; U-98% 15N]	1.2 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	2 mM
5	D2O	natural abundance	10 %
6	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Release date

2008-02-04

Citation

NMR assignment of the domain 513-651 from the SARS-CoV nonstructural protein nsp3
Chatterjee, A., Johnson, M.A., Serrano, P., Pedrini, B., Wuthrich, K.
Biomol. NMR Assign. (2007), 1, 191-194, PubMed 19636862 , DOI 10.1007/s12104-007-9052-x , Abstract

Related entities 1. nsp3(513-651), : 1 : 1 : 2 : 16 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC