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BMRB: 15470

2KDU

Resonance assignment of the Calmodulin-Munc13-1 peptide complex

Authors

Rodriguez-Castaneda, F.A., Carlomagno, T., Brose, N., Griesinger, C.

Assembly

Calmodulin-Munc13-1

Entity

1. calmodulin (polymer, Thiol state: not present), 148 monomers, 16705.23 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREANI DGDGQVNYEE FVQMMTAK

2. Munc13-1 (458-492) (polymer, Thiol state: not present), 36 monomers, 4168.763 Da Detail

GSRAKANWLR AFNKVRMQLQ EARGEGEMSK SLWFKG

3. CA (non-polymer), 40.078 × 4 Da

Total weight

21034.307 Da

Max. entity weight

16705.23 Da

Source organism

Xenopus laevis , Rattus norvegicus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 85.8 %, Completeness (bb): 93.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.8 % (1815 of 2115)	85.6 % (946 of 1105)	83.7 % (677 of 809)	95.5 % (192 of 201)
Backbone	93.7 % (1031 of 1100)	99.0 % (377 of 381)	88.3 % (474 of 537)	98.9 % (180 of 182)
Sidechain	78.1 % (925 of 1184)	78.3 % (567 of 724)	78.7 % (347 of 441)	57.9 % (11 of 19)
Aromatic	72.2 % (104 of 144)	86.1 % (62 of 72)	57.1 % (40 of 70)	100.0 % (2 of 2)
Methyl	92.2 % (153 of 166)	98.8 % (82 of 83)	85.5 % (71 of 83)

1. calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREANI DGDGQVNYEE FVQMMTAK

2. Munc13-1 (458-492)

GSRAKANWLR AFNKVRMQLQ EARGEGEMSK SLWFKG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1), Details uniformly 13C,15N labeled calmodulin bound to the syntethic Munc13-1(459-492) peptide

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1), Details Uniformly 13C,15N labeled Munc13-1 (458-492) peptide bound to calmodulin

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1), Details Uniformly 13C,15N calmodulin bound to a Munc13-1 (459-492) synthetic peptide

#	Name	Isotope labeling	Concentration
11	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
12	Munc13-1	natural abundance	1.8 (±0.2) mM
13	Calcium chloride	natural abundance	10 (±1.0) mM
14	Bis-Tris	natural abundance	20 (±2.0) mM
15	Potassium chloride	natural abundance	150 (±15.0) mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
16	calmodulin	[U-15N]	1.5 (±0.2) mM
17	Munc13-1	natural abundance	1.8 (±0.2) mM
18	Calcium chloride	natural abundance	10 (±1.0) mM
19	Bis-Tris	natural abundance	20 (±2.0) mM
20	Potassium chloride	natural abundance	150 (±15.0) mM
21	Bacteriophage Pf1	natural abundance	4.2 (±0.4) mg

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
22	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
23	calmodulin	natural abundance	0.6 (±0.1) mM
24	Calcium chloride	natural abundance	10 (±1.0) mM
25	Bis-Tris	natural abundance	20 (±2.0) mM
26	Potassium chloride	natural abundance	150 (±15.0) mM
27	Bacteriophage Pf1	natural abundance	4.2 (±0.4) mg

Release date

2009-12-20

Citation

1H, 13C and 15N resonance assignments of the Calmodulin-Munc13-1 peptide complex
Rodriguez-Castaneda, F., Coudevylle, N., Becker, S., Brose, N., Carlomagno, T., Griesinger, C.
Biomol. NMR Assign. (2010), 4, 45-48, PubMed 20013162 , DOI 10.1007/s12104-009-9204-2 , Abstract

Related entities 1. calmodulin, : 11 : 88 entities Detail

Related entities 2. Munc13-1 (458-492), : 1 : 4 : 15 entities Detail

Interaction partners 2. Munc13-1 (458-492), : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 27 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1), Details uniformly 13C,15N labeled calmodulin bound to the syntethic Munc13-1(459-492) peptide

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

2 2D 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz HCN probehead with Z-gradient coil unit

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1), Details Uniformly 13C,15N calmodulin bound to a Munc13-1 (459-492) synthetic peptide

#	Name	Isotope labeling	Concentration
11	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
12	Munc13-1	natural abundance	1.8 (±0.2) mM
13	Calcium chloride	natural abundance	10 (±1.0) mM
14	Bis-Tris	natural abundance	20 (±2.0) mM
15	Potassium chloride	natural abundance	150 (±15.0) mM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

4 3D HNCACB

Instrument

Bruker Avance - 700 MHz HCN probehead with triple-axis gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

5 3D HNCO

Instrument

Bruker Avance - 700 MHz HCN probehead with triple-axis gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

6 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
11	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
12	Munc13-1	natural abundance	1.8 (±0.2) mM
13	Calcium chloride	natural abundance	10 (±1.0) mM
14	Bis-Tris	natural abundance	20 (±2.0) mM
15	Potassium chloride	natural abundance	150 (±15.0) mM

9 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz HCN probehead with triple-axis gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

10 3D C(CO)NH

Instrument

Bruker Avance - 700 MHz HCN probehead with triple-axis gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
2	Munc13-1	natural abundance	1.8 (±0.2) mM
3	Calcium chloride	natural abundance	10 (±1.0) mM
4	Bis-Tris	natural abundance	20 (±2.0) mM
5	Potassium chloride	natural abundance	150 (±15.0) mM

11 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
11	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
12	Munc13-1	natural abundance	1.8 (±0.2) mM
13	Calcium chloride	natural abundance	10 (±1.0) mM
14	Bis-Tris	natural abundance	20 (±2.0) mM
15	Potassium chloride	natural abundance	150 (±15.0) mM

12 2D HBCBCaro

Instrument

Bruker DRX - 600 MHz HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
11	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
12	Munc13-1	natural abundance	1.8 (±0.2) mM
13	Calcium chloride	natural abundance	10 (±1.0) mM
14	Bis-Tris	natural abundance	20 (±2.0) mM
15	Potassium chloride	natural abundance	150 (±15.0) mM

13 3D HMBCMet

Instrument

Bruker Avance - 700 MHz HCN probehead with triple-axis gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
11	calmodulin	[U-13C; U-15N]	1.5 (±0.2) mM
12	Munc13-1	natural abundance	1.8 (±0.2) mM
13	Calcium chloride	natural abundance	10 (±1.0) mM
14	Bis-Tris	natural abundance	20 (±2.0) mM
15	Potassium chloride	natural abundance	150 (±15.0) mM

14 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1), Details Uniformly 13C,15N labeled Munc13-1 (458-492) peptide bound to calmodulin

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

15 2D 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

16 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

17 3D C(CO)NH

Instrument

Bruker DRX - 600 MHz HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

18 3D HNCO

Instrument

Bruker Avance - 900 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

19 3D HNCACB

Instrument

Bruker Avance - 600 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

20 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

21 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

22 3D 1H-15N NOESY

Instrument

Bruker DMX - 800 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

23 3D 1H-13C NOESY

Instrument

Bruker DMX - 800 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

24 3D HN(CA)CO

Instrument

Bruker Avance - 700 MHz HCN probehead with triple-axis gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

25 2D HBCBCaro

Instrument

Bruker DRX - 600 MHz HCN probehead with Z-gradient coil unit

Sample

#	Name	Isotope labeling	Concentration
6	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
7	calmodulin	natural abundance	0.6 (±0.1) mM
8	Calcium chloride	natural abundance	10 (±1.0) mM
9	Bis-Tris	natural abundance	20 (±2.0) mM
10	potassium chloride	natural abundance	150 (±15.0) mM

26 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
16	calmodulin	[U-15N]	1.5 (±0.2) mM
17	Munc13-1	natural abundance	1.8 (±0.2) mM
18	Calcium chloride	natural abundance	10 (±1.0) mM
19	Bis-Tris	natural abundance	20 (±2.0) mM
20	Potassium chloride	natural abundance	150 (±15.0) mM
21	Bacteriophage Pf1	natural abundance	4.2 (±0.4) mg

27 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz Cryogenic cooled HCN probehead with Z-gradient coil unit

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
22	Munc13-1	[U-13C; U-15N]	0.5 (±0.1) mM
23	calmodulin	natural abundance	0.6 (±0.1) mM
24	Calcium chloride	natural abundance	10 (±1.0) mM
25	Bis-Tris	natural abundance	20 (±2.0) mM
26	Potassium chloride	natural abundance	150 (±15.0) mM
27	Bacteriophage Pf1	natural abundance	4.2 (±0.4) mg

Chemical shift validation 5 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15470_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15470_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts	Warning	1962		100.0 (chain: 1, length: 148), 97.2 (chain: 2, length: 36)
1	Chemical shift references	chem_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 148, Sequence coverage: 100.0%
  - Entity_assembly_ID: 2, Chain length: 36, Sequence coverage: 97.2%
- Total number of assignments
  - ¹H chemical shifts: 1105
  - ¹³C chemical shifts: 666
  - ¹⁵N chemical shifts: 191
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	869	738	84.9
¹³C chemical shifts	645	526	81.6
¹⁵N chemical shifts	165	154	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	305	304	99.7
¹³C chemical shifts	296	253	85.5
¹⁵N chemical shifts	146	145	99.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	564	434	77.0
¹³C chemical shifts	349	273	78.2
¹⁵N chemical shifts	19	9	47.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	80	78	97.5
¹³C chemical shifts	80	63	78.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	45	90.0
¹³C chemical shifts	50	34	68.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	236	183	77.5
¹³C chemical shifts	164	140	85.4
¹⁵N chemical shifts	46	37	80.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	76	72	94.7
¹³C chemical shifts	72	70	97.2
¹⁵N chemical shifts	36	35	97.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	160	111	69.4
¹³C chemical shifts	92	70	76.1
¹⁵N chemical shifts	10	2	20.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	14	14	100.0
¹³C chemical shifts	14	13	92.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	17	77.3
¹³C chemical shifts	20	6	30.0
¹⁵N chemical shifts	2	2	100.0

Keywords calmodulin, Munc13, neurotransmitter release, synaptosome, vesicle priming

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Found 1 Document (0.76 seconds)

BMRB: 15470

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Related entities 1. calmodulin, : 11 : 88 entities Detail

Related entities 2. Munc13-1 (458-492), : 1 : 4 : 15 entities Detail

Interaction partners 2. Munc13-1 (458-492), : 2 interactors Detail

Experiments performed 27 experiments Detail

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