Backbone 1H, 13C, and 15N Chemical Shift Assignments for 8M Urea Denatured State of dSUMO-3(dSMT3)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 68.2 % (710 of 1041) | 65.0 % (356 of 548) | 67.5 % (266 of 394) | 88.9 % (88 of 99) |
Backbone | 97.6 % (521 of 534) | 93.5 % (174 of 186) | 99.6 % (260 of 261) | 100.0 % (87 of 87) |
Sidechain | 45.9 % (270 of 588) | 50.3 % (182 of 362) | 40.7 % (87 of 214) | 8.3 % (1 of 12) |
Aromatic | 0.0 % (0 of 54) | 0.0 % (0 of 27) | 0.0 % (0 of 27) | |
Methyl | 40.2 % (33 of 82) | 68.3 % (28 of 41) | 12.2 % (5 of 41) |
1. dSUMO
GSMSDEKKGG ETEHINLKVL GQDNAVVQFK IKKHTPLRKL MNAYCDRAGL SMQVVRFRFD GQPINENDTP TSLEMEEGDT IEVYQQQTGGPressure 1 atm, Temperature 300 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | dSUMO | [U-99% 13C; U-99% 15N] | 1 mM |