1H and 15N resonance assignment of M7, a computationally-designed artificial protein
GSHMKVDITI KIQRDGQEIE IDIRVSTGKE LERALQELEK ALARAGARNV QITISAENDE QAKELLELIA RLLQKLGYKD INVRVNGTEV KIEVRV
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 97.9 % (685 of 700) | 98.0 % (582 of 594) | 97.2 % (103 of 106) |
Backbone | 96.3 % (283 of 294) | 96.0 % (190 of 198) | 96.9 % (93 of 96) |
Sidechain | 99.0 % (402 of 406) | 99.0 % (392 of 396) | 100.0 % (10 of 10) |
Aromatic | 100.0 % (6 of 6) | 100.0 % (6 of 6) | |
Methyl | 100.0 % (70 of 70) | 100.0 % (70 of 70) |
1. M7
GSHMKVDITI KIQRDGQEIE IDIRVSTGKE LERALQELEK ALARAGARNV QITISAENDE QAKELLELIA RLLQKLGYKD INVRVNGTEV KIEVRVSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | M7 | natural abundance | 1.0 mM | |
2 | sodium phosphate | natural abundance | 25 mM | |
3 | sodium azide | natural abundance | 0.05 % | |
4 | H2O | 95 % | ||
5 | D2O | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | M7 | [U-98% 15N] | 1.3 mM | |
7 | sodium phosphate | natural abundance | 25 mM | |
8 | sodium azide | natural abundance | 0.05 % | |
9 | H2O | 95 % | ||
10 | D2O | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | M7 | natural abundance | 1.0 mM | |
12 | sodium phosphate | natural abundance | 25 mM | |
13 | sodium azide | natural abundance | 0.05 % | |
14 | D2O | 100 % |