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BMRB: 15479

2KLU

1H, 13C and 15N Resonance Assignment for the transmembrane and cytoplasmic domains of human CD4

Authors

Wittlich, M., Koenig, B.W., Hoffmann, S., Willbold, D.

Assembly

CD4mut

Entity

1. CD4mut (polymer, Thiol state: not present), 70 monomers, 7850.234 Da Detail

GPLVPRGSMA LIVLGGVAGL LLFIGLGIFF SVRSRHRRRQ AERMSQIKRL LSEKKTSQSP HRFQKTHSPI

Formula weight

7850.234 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.1 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.1 % (832 of 848)	96.9 % (435 of 449)	100.0 % (329 of 329)	97.1 % (68 of 70)
Backbone	98.5 % (406 of 412)	97.2 % (139 of 143)	100.0 % (203 of 203)	97.0 % (64 of 66)
Sidechain	98.0 % (489 of 499)	96.7 % (296 of 306)	100.0 % (189 of 189)	100.0 % (4 of 4)
Aromatic	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. CD4 coreceptor polypeptide

GPLVPRGSMA LIVLGGVAGL LLFIGLGIFF SVRSRHRRRQ AERMSQIKRL LSEKKTSQSP HRFQKTHSPI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 318 K, pH 6.2

#	Name	Isotope labeling	Concentration
1	CD4 coreceptor polypeptide	[U-100% 13C; U-100% 15N]	0.5-1 mM
2	DPC	[U-100% 2H]	200 mM
3	sodium chloride	natural abundance	150 mM
4	sodium phosphate	natural abundance	20 mM
5	sodium azide	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.37 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.37 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 5 Detail

Release date

2008-06-24

Citation

Structural characterization of the transmembrane and cytoplasmic domains of human CD4
Wittlich, M., Koenig, B.W., Hoffmann, S., Willbold, D.
Biochim. Biophys. Acta (2007), 1768, 2949-2960, PubMed 18035040 , DOI 10.1016/j.bbamem.2007.10.023 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16853 released on 2012-08-01
Title NMR structure of the transmembrane and cytoplasmic domains of human CD4

Related entities 1. CD4mut, : 1 : 5 : 23 entities Detail

Interaction partners 1. CD4mut, : 18 interactors Detail

More details for 18 interactors identified by 12 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC