1H assignments of W60G mutant of human beta2-microglobulin
MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD GSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS26:SG | 1:CYS81:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 95.4 % (603 of 632) | 95.4 % (603 of 632) |
Backbone | 98.5 % (196 of 199) | 98.5 % (196 of 199) |
Sidechain | 94.0 % (407 of 433) | 94.0 % (407 of 433) |
Aromatic | 100.0 % (63 of 63) | 100.0 % (63 of 63) |
Methyl | 97.8 % (44 of 45) | 97.8 % (44 of 45) |
1. w60g-b2m
MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD GSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDMSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.6, Details 70 mM phosphate, 100 mM NaCl
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | w60g-b2m | natural abundance | 0.5-0.9 mM | |
2 | phosphate | 70 mM | ||
3 | NaCl | 100 mM | ||
4 | H2O | 95 % | ||
5 | D2O | 5 % |