BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	60.0 % (766 of 1276)	65.6 % (438 of 668)	50.4 % (252 of 500)	70.4 % (76 of 108)
Backbone	56.9 % (361 of 634)	65.4 % (142 of 217)	47.2 % (149 of 316)	69.3 % (70 of 101)
Sidechain	64.2 % (476 of 742)	65.6 % (296 of 451)	61.3 % (174 of 284)	85.7 % (6 of 7)
Aromatic	47.1 % (64 of 136)	61.8 % (42 of 68)	32.4 % (22 of 68)
Methyl	84.8 % (78 of 92)	84.8 % (39 of 46)	84.8 % (39 of 46)

1. Npl3 RRM1

MGSSHHHHHH SSGLVPRGSH MHHRQEGELS NTRLFVRPFP LDVQESELNE IFGPFGPMKE VKILNGFAFV EFEEAESAAK AIEEVHGKSF ANQPLEVVYS KLPAKRYR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Type	Concentration
4	Npl3 RRM1	[U-13C; U-15N]		1 mM
5	D2O			100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
6	Npl3 RRM1	[U-15N]	1 mM
7	H2O		90 %
8	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: 1.88 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: 1.88 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JVO, Strand ID: A Detail

Release date

2008-06-25

Citation

Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L
Skrisovska, L., Allain, F.H-T.
J. Mol. Biol. (2008), 375, 151-164, PubMed 17936301 , DOI 10.1016/j.jmb.2007.09.030 , Abstract

Related entities 1. Npl3 RRM1, : 1 : 4 : 46 entities Detail

Interaction partners 1. Npl3 RRM1, : 63 interactors Detail

More details for 63 interactors identified by 16 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

2 3D HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

3 3D HN(CO)CA

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

4 3D HNCACB

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

5 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

6 3D HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

7 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

8 3D 1H-15N NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

9 2D 1H-1H TOCSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Type	Concentration
4	Npl3 RRM1	[U-13C; U-15N]		1 mM
5	D2O			100 %

10 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Type	Concentration
4	Npl3 RRM1	[U-13C; U-15N]		1 mM
5	D2O			100 %

11 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7

#	Name	Isotope labeling	Concentration
1	Npl3 RRM1	[U-13C; U-15N]	1 mM
2	H2O		90 %
3	D2O		10 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_15485_2jvo.nef
Input source #2: Coordindates	2jvo.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MGSSHHHHHHSSGLVPRGSHMHHRQEGELSNTRLFVRPFPLDVQESELNEIFGPFGPMKEVKILNGFAFVEFEEAESAAKAIEEVHGKSFANQPLEVVYS
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MGSSHHHHHHSSGLVPRGSHMHHRQEGELSNTRLFVRPFPLDVQESELNEIFGPFGPMKEVKILNGFAFVEFEEAESAAKAIEEVHGKSFANQPLEVVYS

--------
KLPAKRYR
||||||||
KLPAKRYR

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	108	0	0	100.0

Content subtype: combined_15485_2jvo.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	743		68.5 (chain: A, length: 108)
1	Distance restraints	AMBER_distance_constraints_2	OK	2179 (1)	noe	66.7 (chain: A, length: 108)
1	Dihedral angle restraints	AMBER_dihedral_4	Warning	1 (1)	.	1.9 (chain: A, length: 108)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 108, Sequence coverage: 68.5%
- Total number of assignments
  - ¹H chemical shifts: 433
  - ¹³C chemical shifts: 237
  - ¹⁵N chemical shifts: 73
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	668	433	64.8
¹³C chemical shifts	500	237	47.4
¹⁵N chemical shifts	114	73	64.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	217	139	64.1
¹³C chemical shifts	216	67	31.0
¹⁵N chemical shifts	101	67	66.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	451	294	65.2
¹³C chemical shifts	284	170	59.9
¹⁵N chemical shifts	13	6	46.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	49	40	81.6
¹³C chemical shifts	49	40	81.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	68	41	60.3
¹³C chemical shifts	68	22	32.4

Distance restraints

Saveframe: AMBER_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 108, Sequence coverage: 66.7%
- Total number of restraints: 2179
- Weight of restraints: 1.0 (2179)
- Potential type of restraints: square-well-parabolic (2179)
- Range of distance target values: 2.05, 5.12 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: AMBER_dihedral_4
- Status: Warning
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 108, Sequence coverage: 1.9%
- Total number of restraints: 1
- Total number of restraints per polymer type: 1
- Weight of restraints: 1.0 (1)
- Potential type of restraints: square-well-parabolic (1)
- Dihedral angle restraints per residue

Keywords EPL, NMR, Segmental labeling, Structural Biology

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Found 1 Document (1.928 seconds)

BMRB: 15485

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Npl3 RRM1, : 1 : 4 : 46 entities Detail

Interaction partners 1. Npl3 RRM1, : 63 interactors Detail

Experiments performed 11 experiments Detail

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NMR combined restraints 4 contents Detail