Chemical Shift Assignments for Legionella pneumophila Mip bound to rapamycin
FNKKADENKV KGEAFLTENK NKPGVVVLPS GLQYKVINSG NGVKPGKSDT VTVEYTGRLI DGTVFDSTEK TGKPATFQVS QVIPGWTEAL QLMPAGSTWE IYVPSGLAYG PRSVGGPIGP NETLIFKIHL ISVKKSS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.1 % (1437 of 1577) | 87.9 % (720 of 819) | 93.7 % (579 of 618) | 98.6 % (138 of 140) |
Backbone | 96.9 % (777 of 802) | 96.1 % (269 of 280) | 96.7 % (382 of 395) | 99.2 % (126 of 127) |
Sidechain | 87.2 % (781 of 896) | 83.7 % (451 of 539) | 92.4 % (318 of 344) | 92.3 % (12 of 13) |
Aromatic | 60.9 % (67 of 110) | 60.0 % (33 of 55) | 60.4 % (32 of 53) | 100.0 % (2 of 2) |
Methyl | 96.2 % (152 of 158) | 94.9 % (75 of 79) | 97.5 % (77 of 79) |
1. Mip protein
FNKKADENKV KGEAFLTENK NKPGVVVLPS GLQYKVINSG NGVKPGKSDT VTVEYTGRLI DGTVFDSTEK TGKPATFQVS QVIPGWTEAL QLMPAGSTWE IYVPSGLAYG PRSVGGPIGP NETLIFKIHL ISVKKSSSolvent system 90 % H2O and 10 % D2O at pH 6.5, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1:1 complex of rapamycin and Mip77-213 dissolved in 20 mM potassium phosphate buffer
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Mip protein | [U-98% 13C; U-98% 15N] | 2 mM | |
2 | Rapamycin | natural abundance | 2 mM |