N-terminal SH3 domain of human Nckalpha
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 57.1 % (485 of 850) | 97.3 % (432 of 444) | 12.7 % (41 of 323) | 14.5 % (12 of 83) |
Backbone | 41.9 % (180 of 430) | 95.9 % (139 of 145) | 14.0 % (30 of 214) | 15.5 % (11 of 71) |
Sidechain | 64.5 % (316 of 490) | 98.0 % (293 of 299) | 12.3 % (22 of 179) | 8.3 % (1 of 12) |
Aromatic | 50.0 % (36 of 72) | 100.0 % (36 of 36) | 0.0 % (0 of 33) | 0.0 % (0 of 3) |
Methyl | 55.7 % (39 of 70) | 100.0 % (35 of 35) | 11.4 % (4 of 35) |
1. Nckalpha-SH3.1
GSTMAEEVVV VAKFDYVAQQ EQELDIKKNE RLWLLDDSKS WWRVRNSMNK TGFVPSNYVE RKNSARAAAN SSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Nckalpha-SH3.1 | natural abundance | 1 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | [U-100% 2H] | 10 % | |
5 | DSS | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Nckalpha-SH3.1, | natural abundance | 1 mM | |
7 | sodium phosphate | natural abundance | 50 mM | |
8 | D2O | [U-100% 2H] | 100 % | |
9 | DSS | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | Nckalpha-SH3.1 | [U-100% 15N] | 1 mM | |
11 | sodium phosphate | natural abundance | 50 mM | |
12 | H2O | natural abundance | 90 % | |
13 | D2O | [U-100% 2H] | 10 % | |
14 | DSS | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | Nckalpha-SH3.1 | [U-100% 13C; U-100% 15N] | 1.2 mM | |
16 | sodium phosphate | natural abundance | 50 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | [U-100% 2H] | 10 % | |
19 | DSS | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Nckalpha-SH3.1 | [U-100% 13C; U-100% 15N] | 1.2 mM | |
21 | sodium phosphate | natural abundance | 50 mM | |
22 | D2O | [U-100% 2H] | 100 % | |
23 | DSS | natural abundance | 0.1 mM |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 62.6 % (1065 of 1700) | 81.0 % (719 of 888) | 39.5 % (255 of 646) | 54.8 % (91 of 166) |
Backbone | 69.2 % (595 of 860) | 96.2 % (279 of 290) | 55.4 % (237 of 428) | 55.6 % (79 of 142) |
Sidechain | 56.0 % (549 of 980) | 73.6 % (440 of 598) | 27.1 % (97 of 358) | 50.0 % (12 of 24) |
Aromatic | 31.9 % (46 of 144) | 56.9 % (41 of 72) | 3.0 % (2 of 66) | 50.0 % (3 of 6) |
Methyl | 40.7 % (57 of 140) | 62.9 % (44 of 70) | 18.6 % (13 of 70) |
1. Nckalpha-SH3.1
GSTMAEEVVV VAKFDYVAQQ EQELDIKKNE RLWLLDDSKS WWRVRNSMNK TGFVPSNYVE RKNSARAAAN SSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Nckalpha-SH3.1 | natural abundance | 1 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | [U-100% 2H] | 10 % | |
5 | DSS | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Nckalpha-SH3.1, | natural abundance | 1 mM | |
7 | sodium phosphate | natural abundance | 50 mM | |
8 | D2O | [U-100% 2H] | 100 % | |
9 | DSS | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | Nckalpha-SH3.1 | [U-100% 15N] | 1 mM | |
11 | sodium phosphate | natural abundance | 50 mM | |
12 | H2O | natural abundance | 90 % | |
13 | D2O | [U-100% 2H] | 10 % | |
14 | DSS | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | Nckalpha-SH3.1 | [U-100% 13C; U-100% 15N] | 1.2 mM | |
16 | sodium phosphate | natural abundance | 50 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | [U-100% 2H] | 10 % | |
19 | DSS | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Nckalpha-SH3.1 | [U-100% 13C; U-100% 15N] | 1.2 mM | |
21 | sodium phosphate | natural abundance | 50 mM | |
22 | D2O | [U-100% 2H] | 100 % | |
23 | DSS | natural abundance | 0.1 mM |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 63.6 % (1621 of 2550) | 77.9 % (1038 of 1332) | 49.2 % (477 of 969) | 42.6 % (106 of 249) |
Backbone | 65.3 % (842 of 1290) | 83.9 % (365 of 435) | 59.7 % (383 of 642) | 44.1 % (94 of 213) |
Sidechain | 62.9 % (924 of 1470) | 75.0 % (673 of 897) | 44.5 % (239 of 537) | 33.3 % (12 of 36) |
Aromatic | 21.3 % (46 of 216) | 38.0 % (41 of 108) | 2.0 % (2 of 99) | 33.3 % (3 of 9) |
Methyl | 59.0 % (124 of 210) | 74.3 % (78 of 105) | 43.8 % (46 of 105) |
1. Nckalpha-SH3.1
GSTMAEEVVV VAKFDYVAQQ EQELDIKKNE RLWLLDDSKS WWRVRNSMNK TGFVPSNYVE RKNSARAAAN SSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Nckalpha-SH3.1 | natural abundance | 1 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | [U-100% 2H] | 10 % | |
5 | DSS | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Nckalpha-SH3.1, | natural abundance | 1 mM | |
7 | sodium phosphate | natural abundance | 50 mM | |
8 | D2O | [U-100% 2H] | 100 % | |
9 | DSS | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | Nckalpha-SH3.1 | [U-100% 15N] | 1 mM | |
11 | sodium phosphate | natural abundance | 50 mM | |
12 | H2O | natural abundance | 90 % | |
13 | D2O | [U-100% 2H] | 10 % | |
14 | DSS | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | Nckalpha-SH3.1 | [U-100% 13C; U-100% 15N] | 1.2 mM | |
16 | sodium phosphate | natural abundance | 50 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | [U-100% 2H] | 10 % | |
19 | DSS | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7, Details 50 mM sodium phosphate buffer, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Nckalpha-SH3.1 | [U-100% 13C; U-100% 15N] | 1.2 mM | |
21 | sodium phosphate | natural abundance | 50 mM | |
22 | D2O | [U-100% 2H] | 100 % | |
23 | DSS | natural abundance | 0.1 mM |