1H, 13C, and 15N NMR chemical shift assignments of the palladin Ig3 domain
GGSNATAPFF EMKLKHYKIF EGMPVTFTCR VAGNPKPKIY WFKDGKQISP KSDHYTIQRD LDGTCSLHTT ASTLDDDGNY TIMAANPQGR VSCTGRLMVQ AVNQRGRS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.8 % (1173 of 1237) | 94.0 % (606 of 645) | 95.0 % (455 of 479) | 99.1 % (112 of 113) |
Backbone | 98.9 % (629 of 636) | 98.2 % (216 of 220) | 99.0 % (311 of 314) | 100.0 % (102 of 102) |
Sidechain | 91.8 % (642 of 699) | 91.8 % (390 of 425) | 92.0 % (242 of 263) | 90.9 % (10 of 11) |
Aromatic | 84.9 % (90 of 106) | 88.7 % (47 of 53) | 80.8 % (42 of 52) | 100.0 % (1 of 1) |
Methyl | 95.7 % (90 of 94) | 95.7 % (45 of 47) | 95.7 % (45 of 47) |
1. Palladin Ig3 domain
GGSNATAPFF EMKLKHYKIF EGMPVTFTCR VAGNPKPKIY WFKDGKQISP KSDHYTIQRD LDGTCSLHTT ASTLDDDGNY TIMAANPQGR VSCTGRLMVQ AVNQRGRSPressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Palladin Ig3 domain | [U-98% 13C; U-98% 15N] | 0.3-1.8 mM | |
2 | D2O | natural abundance | 10 % | |
3 | H2O | natural abundance | 90 % | |
4 | TRIS | natural abundance | 50 mM | |
5 | sodium chloride | natural abundance | 150 mM | |
6 | DTT | natural abundance | 2 mM |