1H and 15N Resonance assignments for the human RLIP76 Ral binding domain
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 95.8 % (413 of 431) | 95.7 % (353 of 369) | 96.8 % (60 of 62) |
Backbone | 95.9 % (165 of 172) | 95.7 % (111 of 116) | 96.4 % (54 of 56) |
Sidechain | 95.8 % (248 of 259) | 95.7 % (242 of 253) | 100.0 % (6 of 6) |
Aromatic | 92.9 % (13 of 14) | 92.3 % (12 of 13) | 100.0 % (1 of 1) |
Methyl | 100.0 % (33 of 33) | 100.0 % (33 of 33) |
1. RLIP76
GSETQAGIKE EIRRQEFLLN SLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREAPressure 1 atm, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RLIP76 | [U-98% 15N] | 0.8 mM | |
2 | D2O | [U-100% 2H] | 10 % | |
3 | sodium phosphate | natural abundance | 50 mM | |
4 | sodium chloride | natural abundance | 100 mM | |
5 | sodium azide | natural abundance | 0.05 % | |
6 | beta-mercaptoethanol | natural abundance | 10 mM | |
7 | magnesium chloride | natural abundance | 1 mM |