Search

Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA
Authors
Stoll, R., Lee, B.M., Debler, E.W., Laity, J.H., Wilson, I.A., Dyson, H.JANE., Wright, P.E.
Assembly
wt1-17mer
Entity
1. wt1-17mer (polymer, Thiol state: all other bound), 119 monomers, 14448.38 Da Detail

ASEKRPFMCA YPGCNKRYFK LSHLQMHSRK HTGEKPYQCD FKDCERRFSR SDQLKRHQRR HTGVKPFQCK TCQRKFSRSD HLKTHTRTHT GEKPFSCRWP SCQKKFARSD ELVRHHNMH


2. ZN (non-polymer), 65.409 × 4 Da
Total weight
14710.016 Da
Max. entity weight
14448.38 Da
Entity Connection
na 16 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS9:SG2:ZN1:ZN
2nasing1:CYS14:SG2:ZN1:ZN
3nasing1:HIS27:NE22:ZN1:ZN
4nasing1:HIS31:NE22:ZN1:ZN
5nasing1:CYS39:SG2:ZN1:ZN
6nasing1:CYS44:SG2:ZN1:ZN
7nasing1:HIS57:NE22:ZN1:ZN
8nasing1:HIS61:NE22:ZN1:ZN
9nasing1:CYS69:SG2:ZN1:ZN
10nasing1:CYS72:SG2:ZN1:ZN
11nasing1:HIS85:NE22:ZN1:ZN
12nasing1:HIS89:NE22:ZN1:ZN
13nasing1:CYS97:SG2:ZN1:ZN
14nasing1:CYS102:SG2:ZN1:ZN
15nasing1:HIS115:NE22:ZN1:ZN
16nasing1:HIS119:NE22:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 91.6 %, Completeness: 25.5 %, Completeness (bb): 63.6 % Detail

Polymer type: polypeptide(L)

Total1H15N
All25.5 % (234 of 917)15.6 % (124 of 794)89.4 % (110 of 123)
Backbone63.6 % (222 of 349)47.9 % (113 of 236)96.5 % (109 of 113)
Sidechain 2.1 % (12 of 568) 2.0 % (11 of 558)10.0 % (1 of 10)
Aromatic 6.2 % (5 of 81) 5.0 % (4 of 80)100.0 % (1 of 1)
Methyl 0.0 % (0 of 24) 0.0 % (0 of 24)

1. wt1-17mer

ASEKRPFMCA YPGCNKRYFK LSHLQMHSRK HTGEKPYQCD FKDCERRFSR SDQLKRHQRR HTGVKPFQCK TCQRKFSRSD HLKTHTRTHT GEKPFSCRWP SCQKKFARSD ELVRHHNMH

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.7


#NameIsotope labelingTypeConcentration
1wt1[U-100% 13C; U-100% 15N]0.6 mM
2Tris/HCl10 mM
3KCl20 mM
4ZnSO45 uM
5NaN32 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 2JP9, Strand ID: A Detail


Release date
2008-02-13
Citation
Structure of the Wilms tumor suppressor protein zinc finger domain bound to DNA
Stoll, R., Lee, B.M., Debler, E.W., Laity, J.H., Wilson, I.A., Dyson, H.JANE., Wright, P.E.
J. Mol. Biol. (2007), 372, 1227-1245, PubMed 17716689 , DOI 10.1016/j.jmb.2007.07.017 ,
Related entities 1. wt1-17mer, : 1 : 2 : 2 : 5 : 428 entities Detail
Interaction partners 1. wt1-17mer, : 14 interactors Detail
Experiments performed 2 experiments Detail
NMR combined restraints 13 contents Detail
Keywords DNA binding, NMR, nucleic acid recognition, residual dipolar coupling, X-ray, zinc finger