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Solution Structure of Human C6orf115 Protein
Authors
Lin, J., Wang, J.
Assembly
c6orf115
Entity
1. c6orf115 (polymer, Thiol state: not present), 89 monomers, 10105.42 Da Detail

MNVDHEVNLL VEEIHRLGSK NADGKLSVKF GVLFRDDKSA NLFEALVGTL KAAKRRKIVT YPGELLLQGV HDDVDIILLQ DLEHHHHHH


Formula weight
10105.42 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.5 %, Completeness: 89.9 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All89.9 % (942 of 1048)90.8 % (491 of 541)87.9 % (363 of 413)93.6 % (88 of 94)
Backbone92.5 % (492 of 532)94.0 % (172 of 183)91.6 % (239 of 261)92.0 % (81 of 88)
Sidechain87.6 % (525 of 599)88.8 % (318 of 358)85.5 % (201 of 235)100.0 % (6 of 6)
Aromatic31.1 % (23 of 74)35.1 % (13 of 37)27.0 % (10 of 37)
Methyl98.4 % (120 of 122)98.4 % (60 of 61)98.4 % (60 of 61)

1. c6orf115

MNVDHEVNLL VEEIHRLGSK NADGKLSVKF GVLFRDDKSA NLFEALVGTL KAAKRRKIVT YPGELLLQGV HDDVDIILLQ DLEHHHHHH

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 4.4


#NameIsotope labelingTypeConcentration
1entity[U-100% 13C; U-100% 15N]1.5 mM
2potassium chloridenatural abundance20 mM
3sodium acetate/acetic acidnatural abundance50 mM
4sodium azidenatural abundance0.05 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 4.4


#NameIsotope labelingTypeConcentration
5entity[U-100% 15N]1.5 mM
6potassium chloridenatural abundance20 mM
7sodium acetate/acetic acidnatural abundance50 mM
8sodium azidenatural abundance0.05 %

LACS Plot; CA
Referencing offset: -0.18 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.18 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.0 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.09 ppm, Outliers: 1 Detail
Release date
2012-08-02
Citation
Solution Structure of Human C6orf115 protein
Lin, J., Wang, J.
Not known
Related entities 1. c6orf115, : 1 : 2 : 42 entities Detail
Interaction partners 1. c6orf115, : 5 interactors Detail
Experiments performed 13 experiments Detail
Chemical shift validation 3 contents Detail
Keywords winged-helix fold