1H, 13C and 15N resonance assignments from the Gal_lectin domain of mouse Latrophilin-1 GPCR
GLPFGLMRRE LACEGYPIEL RCPGSDVIMV ENANYGRTDD KICDADPFQM ENVQCYLPDA FKIMSQRCNN RTQCVVVAGS DAFPDPCPGT YKYLEVQYDC VPYKVE
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | covalent | sing | 1:ASN70:ND2 | 2:NAG1:C1 |
2 | disulfide | sing | 1:CYS13:SG | 1:CYS43:SG |
3 | disulfide | sing | 1:CYS22:SG | 1:CYS100:SG |
4 | disulfide | sing | 1:CYS55:SG | 1:CYS87:SG |
5 | disulfide | sing | 1:CYS68:SG | 1:CYS74:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.2 % (1168 of 1214) | 97.2 % (617 of 635) | 94.5 % (446 of 472) | 98.1 % (105 of 107) |
Backbone | 96.9 % (599 of 618) | 98.6 % (207 of 210) | 95.5 % (297 of 311) | 97.9 % (95 of 97) |
Sidechain | 96.0 % (667 of 695) | 96.5 % (410 of 425) | 95.0 % (247 of 260) | 100.0 % (10 of 10) |
Aromatic | 83.3 % (80 of 96) | 85.4 % (41 of 48) | 81.3 % (39 of 48) | |
Methyl | 95.7 % (90 of 94) | 95.7 % (45 of 47) | 95.7 % (45 of 47) |
1. Gal lectin
GLPFGLMRRE LACEGYPIEL RCPGSDVIMV ENANYGRTDD KICDADPFQM ENVQCYLPDA FKIMSQRCNN RTQCVVVAGS DAFPDPCPGT YKYLEVQYDC VPYKVESolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.02)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Gal_lectin | [U-100% 15N] | 1-2 (±0.1) mM | |
2 | carbohydrate | [U-100% 15N] | 1-2 (±0.1) mM | |
3 | D2O | natural abundance | 5 % | |
4 | sodium azide | natural abundance | 0.02 % | |
5 | sodium phosphate | natural abundance | 20 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.02)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Gal_lectin | [U-100% 13C; U-100% 15N] | 1-2 (±0.1) mM | |
7 | carbohydrate | [U-100% 13C; U-100% 15N] | 1-2 (±0.1) mM | |
8 | D2O | natural abundance | 5 % | |
9 | sodium phosphate | natural abundance | 20 mM | |
10 | sodium azide | natural abundance | 0.02 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.02)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | Gal_lectin | [U-100% 13C; U-100% 15N] | 1-2 (±0.1) mM | |
12 | carbohydrate | [U-100% 13C; U-100% 15N] | 1-2 (±0.1) mM | |
13 | D2O | natural abundance | 100 % | |
14 | sodium azide | natural abundance | 0.02 % | |
15 | sodium phosphate | natural abundance | 20 mM |