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BMRB: 15557

2KVK

1H, 13C, and 15N Chemical Shift Assignment for actin depolymerizing and dynamics regulatory protein from Leishmania donovani

Authors

Pathak, P., Pulavarti, S., Jain, A., Sahasrabuddhe, A., Gupta, C., Arora, A.

Assembly

ADF monomer

Entity

1. ADF monomer (polymer, Thiol state: all free), 150 monomers, 17053.18 Da Detail

MAISGVTLEE SVRGAIDDLR MKKSRYVMMC IGADGKKIEV TEVGERSVNY TDLKEKFSTE KPCYVAFDFE YNDAGSKREK LILIQWIPDT ARPREKMMYS ASRDALSSVS EGYLPIQAND ESGLDAEEII RKVRLHRSVA AALEHHHHHH

Formula weight

17053.18 Da

Source organism

Leishmania donovani

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 84.4 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.4 % (1461 of 1732)	82.8 % (748 of 903)	84.5 % (572 of 677)	92.8 % (141 of 152)
Backbone	92.9 % (829 of 892)	90.8 % (276 of 304)	94.3 % (417 of 442)	93.2 % (136 of 146)
Sidechain	77.3 % (759 of 982)	78.1 % (468 of 599)	75.9 % (286 of 377)	83.3 % (5 of 6)
Aromatic	3.4 % (4 of 118)	6.8 % (4 of 59)	0.0 % (0 of 58)	0.0 % (0 of 1)
Methyl	92.3 % (144 of 156)	92.3 % (72 of 78)	92.3 % (72 of 78)

1. Actin Depolymerzing Factor

MAISGVTLEE SVRGAIDDLR MKKSRYVMMC IGADGKKIEV TEVGERSVNY TDLKEKFSTE KPCYVAFDFE YNDAGSKREK LILIQWIPDT ARPREKMMYS ASRDALSSVS EGYLPIQAND ESGLDAEEII RKVRLHRSVA AALEHHHHHH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Actin Depolymerzing Factor	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.1 %
5	DTT	natural abundance	1 mM
6	D2O	natural abundance	5 %
7	AEBSF protease inhibitor	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KVK, Strand ID: A Detail

Release date

2009-11-19

Citation

NMR assignment of actin depolymerizing and dynamics regulatory protein from Leishmania donovani
Pathak, P., Pulavarti, S., Jain, A., Sahasrabuddhe, A., Gupta, C., Arora, A.
Biomol. NMR Assign. (2009), 3, 265-267, PubMed 19844807 , DOI 10.1007/s12104-009-9190-4 , Abstract

Related entities 1. ADF monomer, : 1 : 1 : 130 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC