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BMRB: 15577

2L49

C Repressor

Authors

Massad, T., Papadopoulos, E., Damberg, P.

Assembly

Crepressor

Entity

1. Crepressor (polymer, Thiol state: not present), 99 monomers, 11017.32 Da Detail

MSNTISEKIV LMRKSEYLSR QQLADLTGVP YGTLSYYESG RSTPPTDVMM NILQTPQFTK YTLWFMTNQI APESGQIAPA LAHFGQNETT SPHSGQKTG

Formula weight

11017.32 Da

Source organism

Escherichia virus P2

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.9 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.9 % (1077 of 1147)	93.3 % (556 of 596)	93.5 % (417 of 446)	99.0 % (104 of 105)
Backbone	96.9 % (562 of 580)	96.0 % (190 of 198)	96.9 % (281 of 290)	98.9 % (91 of 92)
Sidechain	92.0 % (606 of 659)	92.0 % (366 of 398)	91.5 % (227 of 248)	100.0 % (13 of 13)
Aromatic	77.8 % (70 of 90)	80.0 % (36 of 45)	75.0 % (33 of 44)	100.0 % (1 of 1)
Methyl	96.9 % (95 of 98)	98.0 % (48 of 49)	95.9 % (47 of 49)

1. Crepressor

MSNTISEKIV LMRKSEYLSR QQLADLTGVP YGTLSYYESG RSTPPTDVMM NILQTPQFTK YTLWFMTNQI APESGQIAPA LAHFGQNETT SPHSGQKTG

Show sample condition

Sample

Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -0.16 ppm, Outliers: 2 Detail

Release date

2008-10-13

Citation

Assignment of 1H, 13C, and 15N chemical shift resonances of P2 C-repressor protein
Massad, T., Papadopoulos, E., Henriksson-Peltola, P., Haggard-Ljungquist, E., Damberg, P.
Biomol. NMR Assign. (2008), 2, 215-217, PubMed 19636908 , DOI 10.1007/s12104-008-9124-6 , Abstract

Related entities 1. Crepressor, : 1 : 1 : 1 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

4 3D HNCO

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

5 3D HNCA

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

6 3D HNCACB

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

7 3D HCCH-TOCSY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

8 3D HNHA

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 0.7 atm, Temperature 310 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Crepressor	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		5 mM
3	D2O	[U-100% 2H]		10 %
4	H2O			90 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15577_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15577_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1198		100.0 (chain: 1, length: 99)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 99, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 670
  - ¹³C chemical shifts: 422
  - ¹⁵N chemical shifts: 106
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1