BMRBj - BMREntryMaster

Found 1 Document (0.427 seconds)

BMRB: 15586

2JUT

alpha-RgIA, a Novel Conotoxin that Blocks the nAChR

Authors

Feng, Z., Ellison, M., Park, A., Zhang, X., McIntosh, M., Olivera, B., Norton, R.

Assembly

toxin

Entity

1. toxin (polymer, Thiol state: all disulfide bound), 13 monomers, 1489.727 Da Detail

GCCSDPRCAY RCR

Formula weight

1489.727 Da

Source organism

Conus regius

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.6 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	98.6 % (71 of 72)	98.6 % (71 of 72)
Backbone	96.2 % (25 of 26)	96.2 % (25 of 26)
Sidechain	100.0 % (46 of 46)	100.0 % (46 of 46)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. RgIA

GCCSDPRCAY RCR

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.1

#	Name	Isotope labeling	Concentration
1	RgIA	natural abundance	0.6 mM
2	D2O	[U-100% 2H]	5 %
3	H2O		95 %

Release date

2008-03-12

Citation

Alpha-RgIA, a novel conotoxin that blocks the alpha9alpha10 nAChR: structure and identification of key receptor-binding residues
Ellison, M., Feng, Z., Park, A., Zhang, X., McIntosh, M., Olivera, B., Norton, R.
J. Mol. Biol. (2008), 377, 1216-1227, PubMed 18295795 , DOI 10.1016/j.jmb.2008.01.082 , Abstract

Related entities 1. toxin, : 1 : 5 : 23 entities Detail

Interaction partners 1. toxin, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	74		100.0 (chain: 1, length: 13)
1	Chemical shift references	chemical_shift_reference_1	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
10	TYR	HH	9.82

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	72	71	98.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	26	25	96.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	46	46	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1	1	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	4	100.0

Keywords conotoxin, nicotinic acetylcholine receptor, NMR, pain, peptide, structure

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15586_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (0.427 seconds)

BMRB: 15586

Sample

Related entities 1. toxin, : 1 : 5 : 23 entities Detail

Interaction partners 1. toxin, : 1 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail