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BMRB: 15591

2JY7

NMR structure of the ubiquitin associated (UBA) domain of p62 (SQSTM1). RDC refined

Authors

Long, J.E., Layfield, R., Searle, M.S.

Assembly

P62 UBA

Entity

1. P62 UBA (polymer, Thiol state: not present), 52 monomers, 5739.363 Da Detail

GSPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KH

Formula weight

5739.363 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.9 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.9 % (556 of 592)	91.2 % (281 of 308)	97.0 % (223 of 230)	96.3 % (52 of 54)
Backbone	95.8 % (293 of 306)	95.3 % (101 of 106)	96.0 % (145 of 151)	95.9 % (47 of 49)
Sidechain	92.8 % (309 of 333)	89.1 % (180 of 202)	98.4 % (124 of 126)	100.0 % (5 of 5)
Aromatic	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (20 of 20)	100.0 % (1 of 1)
Methyl	94.2 % (49 of 52)	92.3 % (24 of 26)	96.2 % (25 of 26)

1. P62 UBA

GSPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.04 %
5	D2O	[U-100% 2H]		10 %
6	H2O			90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 5% Polyacrylamide gel

#	Name	Isotope labeling	Type	Concentration
7	entity	[U-100% 15N]		1 mM
8	potassium phosphate	natural abundance		50 mM
9	sodium chloride	natural abundance		50 mM
10	sodium azide	natural abundance		0.04 %
11	D2O	[U-100% 2H]		10 %
12	H2O			90 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
13	entity	[U-100% 15N]		1 mM
14	potassium phosphate	natural abundance		50 mM
15	sodium chloride	natural abundance		50 mM
16	sodium azide	natural abundance		0.04 %
17	D2O	[U-100% 2H]		10 %
18	H2O			90 %

Protein Blocks Logo

Calculated from 30 models in PDB: 2JY7, Strand ID: A Detail

RDC

45 RDC values in 1 lists
Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 7 Detail

Release date

2008-01-01

Citation

Ubiquitin recognition by the ubiquitin-associated domain of p62 involves a novel conformational switch
Long, J.E., Gallagher, T.R., Cavey, J.R., Sheppard, P.W., Ralston, S.H., Layfield, R., Searle, M.S.
J. Biol. Chem. (2008), 283, 5427-5440, PubMed 18083707 , DOI 10.1074/jbc.M704973200 , Abstract

Related entities 1. P62 UBA, : 1 : 4 : 9 : 11 entities Detail

Interaction partners 1. P62 UBA, : 119 interactors Detail

More details for 119 interactors identified by 51 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
13	entity	[U-100% 15N]		1 mM
14	potassium phosphate	natural abundance		50 mM
15	sodium chloride	natural abundance		50 mM
16	sodium azide	natural abundance		0.04 %
17	D2O	[U-100% 2H]		10 %
18	H2O			90 %

2 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
13	entity	[U-100% 15N]		1 mM
14	potassium phosphate	natural abundance		50 mM
15	sodium chloride	natural abundance		50 mM
16	sodium azide	natural abundance		0.04 %
17	D2O	[U-100% 2H]		10 %
18	H2O			90 %

3 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
13	entity	[U-100% 15N]		1 mM
14	potassium phosphate	natural abundance		50 mM
15	sodium chloride	natural abundance		50 mM
16	sodium azide	natural abundance		0.04 %
17	D2O	[U-100% 2H]		10 %
18	H2O			90 %

4 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.04 %
5	D2O	[U-100% 2H]		10 %
6	H2O			90 %

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.04 %
5	D2O	[U-100% 2H]		10 %
6	H2O			90 %

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.04 %
5	D2O	[U-100% 2H]		10 %
6	H2O			90 %

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.04 %
5	D2O	[U-100% 2H]		10 %
6	H2O			90 %

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.04 %
5	D2O	[U-100% 2H]		10 %
6	H2O			90 %

9 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.04 %
5	D2O	[U-100% 2H]		10 %
6	H2O			90 %

10 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.04 %
5	D2O	[U-100% 2H]		10 %
6	H2O			90 %

11 2D 1H-15N HSQC-IPAP

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 5% Polyacrylamide gel

#	Name	Isotope labeling	Type	Concentration
7	entity	[U-100% 15N]		1 mM
8	potassium phosphate	natural abundance		50 mM
9	sodium chloride	natural abundance		50 mM
10	sodium azide	natural abundance		0.04 %
11	D2O	[U-100% 2H]		10 %
12	H2O			90 %

nullKeywords helical bundle, rearrangement, ubiquitin binding, three helices, ubiquitin associated domain