SOLUTION STRUCTURE OF THE BIS-C2-2-NAPHTHYLPYRROLO[2,1-c][1,4]BENZODIAZEPINE (DA046) DNA ADDUCT: THE MOLECULAR BASIS FOR DNA HELIX STABILIZATION.
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 79.9 % (107 of 134) | 79.9 % (107 of 134) |
Suger, PO4 | 75.5 % (74 of 98) | 75.5 % (74 of 98) |
Nucleobase | 91.7 % (33 of 36) | 91.7 % (33 of 36) |
Aromatic | 92.9 % (26 of 28) | 92.9 % (26 of 28) |
Methyl | 100.0 % (6 of 6) | 100.0 % (6 of 6) |
1. DNA AATCTTTAAAGATT
AATCTTTAAA GATTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.85, Details The 0.68 mM DNA solution was incubated with PZD(7.5 mg as a slurry) at room temperature for 48 h under gentle shaking. The excess PZD was removed by filtration (Millipore 25 mm syringe filters, 0.45 m pore size) and the filtrated was collected in the NMR tube. This purification method was verified by repeating the procedure with PZD in D2O ,where filtrate showed no peaks even with higher number of scans (1048).
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*(DA)P*(DA)P*(DT)P*(DC)P*(DT)P*(DT)P*(DT)P*(DA)P*(DA)P*(DA)P*(DG)P*(DA)P*(DT)P*(DT))-3') | natural abundance | 0.68 mM | |
2 | PZD_1 | 0.68 mM | ||
3 | sodium phosphate | 10 mM | ||
4 | sodium chloride | 100 mM | ||
5 | D2O | [U-100% 2H] | 10 % | |
6 | H2O | 90 % |