Solution structure of C-terminal effector domain of putative two-component-system response regulator involved in copper resistance from Klebsiella pneumoniae
MAATVCTIAD MTVDMVRRTV IRSGKKIHLT GKEYVLLELL LQRTGEVLPR SLISSLVWNM NFDSDTNVID VAVRRLRSKI DDDFEPKLIH TVRGAGYVLE IREELEHHHH HH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.8 % (1022 of 1313) | 75.8 % (513 of 677) | 78.7 % (410 of 521) | 86.1 % (99 of 115) |
Backbone | 87.6 % (585 of 668) | 87.7 % (199 of 227) | 87.9 % (291 of 331) | 86.4 % (95 of 110) |
Sidechain | 70.9 % (533 of 752) | 69.8 % (314 of 450) | 72.4 % (215 of 297) | 80.0 % (4 of 5) |
Aromatic | 0.0 % (0 of 80) | 0.0 % (0 of 40) | 0.0 % (0 of 39) | 0.0 % (0 of 1) |
Methyl | 93.7 % (148 of 158) | 94.9 % (75 of 79) | 92.4 % (73 of 79) |
1. TCS C-domain
MAATVCTIAD MTVDMVRRTV IRSGKKIHLT GKEYVLLELL LQRTGEVLPR SLISSLVWNM NFDSDTNVID VAVRRLRSKI DDDFEPKLIH TVRGAGYVLE IREELEHHHH HHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | C-terminal domain of TCS response regulator | [U-13C; U-15N] | 1 mM | |
2 | Tris | natural abundance | 20 mM | |
3 | NaCl | natural abundance | 150 mM | |
4 | Glu | natural abundance | 150 mM | |
5 | Arg | natural abundance | 150 mM |