BMRBj - BMREntryMaster

Found 1 Document (0.369 seconds)

BMRB: 15666

2K0D

Mutant Collision E7 protein IM7 with an extended helix III in M Urea

Authors

Figueiredo, A.MMP., Whittaker, S.B., Knowling, S., Radford, S.E., Moore, G.R.

Assembly

Im7 mutant

Entity

1. Im7 mutant (polymer, Thiol state: not present), 101 monomers, 11076.13 Da Detail

MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGTAL IYEAAARAAA NPGGDGGGPE GIVKEIKEWR AANGKPGFKQ G

Formula weight

11076.13 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 34.2 %, Completeness (bb): 62.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	34.2 % (388 of 1136)	17.7 % (104 of 587)	43.8 % (195 of 445)	85.6 % (89 of 104)
Backbone	62.0 % (371 of 598)	44.2 % (92 of 208)	64.8 % (190 of 293)	91.8 % (89 of 97)
Sidechain	16.4 % (103 of 629)	3.4 % (13 of 379)	37.0 % (90 of 243)	0.0 % (0 of 7)
Aromatic	4.4 % (4 of 90)	4.4 % (2 of 45)	4.5 % (2 of 44)	0.0 % (0 of 1)
Methyl	14.8 % (16 of 108)	3.7 % (2 of 54)	25.9 % (14 of 54)

1. Im7 mutant

MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGTAL IYEAAARAAA NPGGDGGGPE GIVKEIKEWR AANGKPGFKQ G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, Details Im7 mutant in 6 M UREA

#	Name	Isotope labeling	Concentration
1	Im7 mutant	[U-13C; U-15N]	1 mM
2	H2O		95 %
3	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Release date

2009-11-09

Citation

Amino acid insertion reveals a necessary three-helical intermediate in the folding pathway of the colicin E7 immunity protein Im7
Knowling, S.E., Figueiredo, A., Whittaker, S., Moore, G.R., Radford, S.E.
J. Mol. Biol. (2009), 392, 1074-1086, PubMed 19651139 , DOI 10.1016/j.jmb.2009.07.085 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 15645 released on 2009-10-13
Title NMR STRUCTURE OF A MUTANT COLICIN E7 IMMUNITY PROTEIN IM7 WITH AN EXTENDED HELIX III

Related entities 1. Im7 mutant, : 1 : 50 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15666_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15666_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	351		92.1 (chain: 1, length: 101)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 101, Sequence coverage: 92.1%
- Total number of assignments
  - ¹H chemical shifts: 88
  - ¹³C chemical shifts: 175
  - ¹⁵N chemical shifts: 88
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	55	0	0.0
¹³C chemical shifts	55	12	21.8

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Found 1 Document (0.369 seconds)

BMRB: 15666

Sample

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Im7 mutant, : 1 : 50 entities Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	587	88	15.0
¹³C chemical shifts	445	175	39.3
¹⁵N chemical shifts	106	88	83.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	208	88	42.3
¹³C chemical shifts	202	92	45.5
¹⁵N chemical shifts	97	88	90.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	379	0	0.0
¹³C chemical shifts	243	83	34.2
¹⁵N chemical shifts	9	0	0.0