3D NMR structure of domain cC0 of cardiac myosin bonding protein C (MyBP-C)
PEPGKKPVSA FSKKPRSVEV AAGSPAVFEA ETERAGVKVR WQRGGSDISA SNKYGLATEG TRHTLTVREV GPADQGSYAV IAGSSKVKFD LKVIE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.9 % (903 of 1051) | 93.6 % (512 of 547) | 73.5 % (302 of 411) | 95.7 % (89 of 93) |
Backbone | 82.6 % (461 of 558) | 95.9 % (186 of 194) | 68.4 % (188 of 275) | 97.8 % (87 of 89) |
Sidechain | 90.5 % (523 of 578) | 92.4 % (326 of 353) | 88.2 % (195 of 221) | 50.0 % (2 of 4) |
Aromatic | 87.1 % (54 of 62) | 100.0 % (31 of 31) | 73.3 % (22 of 30) | 100.0 % (1 of 1) |
Methyl | 98.0 % (98 of 100) | 100.0 % (50 of 50) | 96.0 % (48 of 50) |
1. cC0 MyBPC
PEPGKKPVSA FSKKPRSVEV AAGSPAVFEA ETERAGVKVR WQRGGSDISA SNKYGLATEG TRHTLTVREV GPADQGSYAV IAGSSKVKFD LKVIESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cC0_MyBPC | [U-95% 13C; U-95% 15N] | 1-1.5 (±0.2) mM | |
2 | D2O | [U-2H] | 55.5 M | |
3 | sodium phosphate | natural abundance | 40 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | DTT | natural abundance | 2 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | cC0_MyBPC | [U-95% 15N] | 1-1.5 (±0.2) mM | |
7 | H2O | natural abundance | 55.5 M | |
8 | sodium phosphate | natural abundance | 40 mM | |
9 | sodium chloride | natural abundance | 50 mM | |
10 | DTT | natural abundance | 2 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | cC0_MyBPC | natural abundance | 1-1.5 (±0.2) mM | |
12 | H2O | natural abundance | 55.5 M | |
13 | sodium phosphate | natural abundance | 40 mM | |
14 | sodium chloride | natural abundance | 50 mM | |
15 | DTT | natural abundance | 2 mM |