Backbone 1H, 13C, and 15N Chemical Shift Assignments for Epidermal Growth Factor-like 2 domain from Microneme Protein 6 (Toxoplasma gondii)
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS14:SG | 1:CYS27:SG |
2 | disulfide | sing | 1:CYS19:SG | 1:CYS36:SG |
3 | disulfide | sing | 1:CYS38:SG | 1:CYS54:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.5 % (552 of 631) | 86.5 % (282 of 326) | 89.5 % (214 of 239) | 84.8 % (56 of 66) |
Backbone | 91.7 % (330 of 360) | 89.7 % (113 of 126) | 93.2 % (164 of 176) | 91.4 % (53 of 58) |
Sidechain | 84.3 % (274 of 325) | 85.0 % (170 of 200) | 86.3 % (101 of 117) | 37.5 % (3 of 8) |
Aromatic | 69.2 % (18 of 26) | 76.9 % (10 of 13) | 61.5 % (8 of 13) | |
Methyl | 82.7 % (43 of 52) | 84.6 % (22 of 26) | 80.8 % (21 of 26) |
1. Epidermal Growth Factor-like domain 2
MANFVQLSET PAACSSNPCG PEAAGTCKET NSGYICRCNQ GYRISLDGTG NVTCIVRQES GSolvent system 90% H2O/10% D2O, Temperature 303 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Epidermal Growth Factor-like domain 2 | [U-98% 13C; U-98% 15N] | 0.5 mM | |
2 | potassium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 50 mM |