BMRB: 15692

Backbone assignment of MIC1-CT from Toxoplasma gondii in complex with EGF3 from TgMIC6

Authors

Saouros, S., Sawmynaden, K., Simpson, P., Matthews, S.

Assembly

TgMIC1 in complex with TgMIC6-EGF3

Entity

1. TgMIC1-CT (polymer, Thiol state: all disulfide bound), 138 monomers, 14151.67 Da Detail

MKTEIHGDST KATLEEGQQL TLTFISTKLD VAVGSCHSLV ANFLDGFLKF QTGSNSAFDV VEVEEPAGPA VLTIGLGHKG RLAVVLDYTR LNAALGSAAY VVEDSGCSSS EEVSFQGVGS GATLVVTTLG ESPTAVSA

2. TgMIC6-EGF3 (polymer, Thiol state: not reported), 69 monomers, 7461.461 Da Detail

IVRQESGCEE NGCGPPDAVQ SCRRLTGTAG RLCVCKENFI ATIDASAHIT CKRVPPHYRK PPFEFGKGG

Total weight

21613.13 Da

Max. entity weight

14151.67 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS36:SG	1:CYS107:SG

Source organism

Toxoplasma gondii

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 64.7 %, Completeness: 40.9 %, Completeness (bb): 58.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	40.9 % (908 of 2220)	36.9 % (418 of 1133)	42.3 % (371 of 878)	56.9 % (119 of 209)
Backbone	58.9 % (721 of 1224)	56.8 % (243 of 428)	60.2 % (360 of 598)	59.6 % (118 of 198)
Sidechain	25.4 % (300 of 1180)	24.8 % (175 of 705)	26.7 % (124 of 464)	9.1 % (1 of 11)
Aromatic	6.7 % (9 of 134)	13.4 % (9 of 67)	0.0 % (0 of 67)
Methyl	19.8 % (49 of 248)	21.0 % (26 of 124)	18.5 % (23 of 124)

1. TgMIC1-CT

MKTEIHGDST KATLEEGQQL TLTFISTKLD VAVGSCHSLV ANFLDGFLKF QTGSNSAFDV VEVEEPAGPA VLTIGLGHKG RLAVVLDYTR LNAALGSAAY VVEDSGCSSS EEVSFQGVGS GATLVVTTLG ESPTAVSA

2. TgMIC6-EGF3

IVRQESGCEE NGCGPPDAVQ SCRRLTGTAG RLCVCKENFI ATIDASAHIT CKRVPPHYRK PPFEFGKGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
1	TgMIC1-CT	[U-100% 13C; U-100% 15N]		1-1.3 mM
2	TgMIC6-EGF3	[U-100% 13C; U-100% 15N]		1-1.3 mM

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LACS Plot; CA

Referencing offset: -0.26 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	0.0 ppm	internal	indirect	1.0

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LACS Plot; CB

Referencing offset: -0.26 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	0.0 ppm	internal	indirect	1.0

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LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	0.0 ppm	internal	indirect	1.0

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LACS Plot; CO

Referencing offset: 0.92 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	0.0 ppm	internal	indirect	1.0

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Release date

2008-10-14

Citation

Complete resonance assignment of the galectin-like domain of MIC1 from Toxoplasma gondii in complex with the second EGF domain from MIC6 and the backbone assignment in complex with the third EGF domain
Saouros, S., Sawmynaden, K., Marchant, J., Simpson, P., Matthews, S.
Biomol. NMR Assign. (2008), 2, 175-177, PubMed 19636898 , DOI 10.1007/s12104-008-9114-8 , Abstract

Related entities 1. TgMIC1-CT, : 1 entities Detail

Related entities 2. TgMIC6-EGF3, : 1 : 2 entities Detail

Interaction partners 1. TgMIC1-CT, : 1 interactors Detail

Interaction partners 2. TgMIC6-EGF3, : 1 interactors Detail

Experiments performed 6 experiments Detail

Chemical shift validation 4 contents Detail