BMRBj - BMREntryMaster

Found 1 Document (0.298 seconds)

BMRB: 15697

2KD8

Mutational and Structural Analysis of Stem-loop IIId of the Hepatitis C Virus and GB Virus B Internal Ribosome Entry Sites

Authors

Thiviyanathan, V., Kulasegaran, R., Kaluarachchi, K., Gorenstein, D.

Assembly

IRES domain IIId

Entity

1. IRES domain IIId (polymer, Thiol state: not present), 22 monomers, 7170.205 Da Detail

GGAUGGUUGG GGUUAGCCAU CC

Formula weight

7170.205 Da

Source organism

Hepatitis GB virus B

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.0 %, Completeness (bb): 88.6 % Detail

Polymer type: polyribonucleotide

	Total	¹H
All	90.0 % (171 of 190)	90.0 % (171 of 190)
Suger, PO4	88.6 % (117 of 132)	88.6 % (117 of 132)
Nucleobase	93.1 % (54 of 58)	93.1 % (54 of 58)
Aromatic	92.0 % (46 of 50)	92.0 % (46 of 50)

1. GBV B IRES

GGAUGGUUGG GGUUAGCCAU CC

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	GBV_B_IRES	natural abundance	1.2 mM
2	potassium chloride	natural abundance	10 mM
3	sodium phosphate	natural abundance	10 mM
4	EDTA	natural abundance	0.05 mM

Release date

2009-10-12

Citation

Mutational and Structural Analysis of Stem-loop IIId of the Hepatitis C Virus and GB Virus B Internal Ribosome Entry Sites
Thiviyanathan, V., Kulasegaran, R., Kaluarachchi, K., Rijnbrand, R., Gorenstein, D.
J. Virology

Related entities 1. IRES domain IIId, : 1 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	179		100.0 (chain: 1, length: 22)
1	Chemical shift references	chemical_shift_reference_1	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
2	G	H21	6.28
2	G	H22	6.28
5	G	H21	6.85
5	G	H22	5.86
6	G	H21	6.23
6	G	H22	5.47
19	A	H61	7.81
19	A	H62	7.81

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	190	171	90.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	132	117	88.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	58	54	93.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	15	15	100.0

Keywords GBV-B, HCV, IRES

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15697_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

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Found 1 Document (0.298 seconds)

BMRB: 15697

Sample

Related entities 1. IRES domain IIId, : 1 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail