BMRB: 15705

Pseudo Contact shifts and RDCs of Galectin-3 with C-terminal LBT tagged at a proton frequency of 600MHz.

Authors

Zhuang, T., Lee, H., Imperiali, B., Prestegard, J.

Assembly

Galectin-3-LBT

Entity

1. Galectin-3-LBT (polymer, Thiol state: all free), 155 monomers, 17726.04 Da Detail

MLIVPYNLPL PGGVVPRMLI TILGTVKPNA NRIALDFQRG NDVAFHFNPR FNERNRRVIV CNTKLDNNWG REERQSVFPF ESGKPFKIQV LVEPDHFKVA VNDAHLLQYN HRVKKLNEIS KLGISGDIDL TSASYTMIYI DTNNDGWYEG DELLA

2. DY (non-polymer), 162.500 Da

Total weight

17888.54 Da

Max. entity weight

17726.04 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 21.9 %, Completeness: 3.5 %, Completeness (bb): 10.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	3.5 % (34 of 970)	3.5 % (34 of 970)
Backbone	10.9 % (34 of 311)	10.9 % (34 of 311)
Sidechain	0.0 % (0 of 659)	0.0 % (0 of 659)
Aromatic	0.0 % (0 of 80)	0.0 % (0 of 80)
Methyl	0.0 % (0 of 91)	0.0 % (0 of 91)

1. Galectin-3-LBT

MLIVPYNLPL PGGVVPRMLI TILGTVKPNA NRIALDFQRG NDVAFHFNPR FNERNRRVIV CNTKLDNNWG REERQSVFPF ESGKPFKIQV LVEPDHFKVA VNDAHLLQYN HRVKKLNEIS KLGISGDIDL TSASYTMIYI DTNNDGWYEG DELLA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 5.4, Details 0.3mM 15N-labeled protein in 50mM MES,50mM NaCl, pH=5.4 mixing with 0.3mM Dysprosium ion

#	Name	Isotope labeling	Type	Concentration
1	Galectin-3-LBT	[U-15N]		0.3 mM
2	MES	[U-100% 2H]		50 mM
3	sodium chloride	natural abundance		50 mM
4	Dysprosium chloride	natural abundance		0.3 mM

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RDC

37 RDC values in 1 lists
Field strength (¹H) 600 MHz, Temperature 298 K, pH 5.4 Detail

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Temperature 298 K, pH 5.4

Experiment name 2D 1H-15N HSQC

List #1 RDC_list_1, RDC code DHN, Field strength (¹H) 600 MHz

Release date

2008-06-29

Citation

Structure determination of a Galectin-3-carbohydrate complex using paramagnetism-based NMR constraints
Zhuang, T., Lee, H., Imperiali, B., Prestegard, J.H.
Protein Sci. (2008), 17, 1220-1231, PubMed 18413860 , DOI 10.1110/ps.034561.108 , Abstract

Related entities 1. Galectin-3-LBT, : 1 : 101 : 158 entities Detail

Interaction partners 1. Galectin-3-LBT, : 62 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail