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BMRB: 15726

2VOP

Backbone 1H, 13C, and 15N Chemical Shift Assignments for La NTD

Authors

Conte, M.R., Sanfelice, D., Kelly, G., Curry, S.

Assembly

La NTD apo

Entity

1. La NTD apo (polymer, Thiol state: all free), 197 monomers, 22983.04 Da Detail

MAENGDNEKM AALEAKICHQ IEYYFGDFNL PRDKFLKEQI KLDEGWVPLE IMIKFNRLNR LTTDFNVIVE ALSKSKAELM EISEDKTKIR RSPSKPLPEV TDEYKNDVKN RSVYIKGFPT DATLDDIKEW LEDKGQVLNI QMRRTLHKAF KGSIFVVFDS IESAKKFVET PGQKYKETDL LILFKDDYFA KKNEVDS

Formula weight

22983.04 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 45.8 %, Completeness (bb): 85.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.8 % (1113 of 2431)	33.5 % (428 of 1279)	54.0 % (510 of 945)	84.5 % (175 of 207)
Backbone	85.0 % (993 of 1168)	83.8 % (330 of 394)	83.7 % (489 of 584)	91.6 % (174 of 190)
Sidechain	18.2 % (265 of 1453)	11.1 % (98 of 885)	30.1 % (166 of 551)	5.9 % (1 of 17)
Aromatic	3.0 % (6 of 200)	6.0 % (6 of 100)	0.0 % (0 of 98)	0.0 % (0 of 2)
Methyl	19.4 % (40 of 206)	17.5 % (18 of 103)	21.4 % (22 of 103)

1. La protein

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7

#	Name	Isotope labeling	Concentration
1	La protein	[U-100% 13C; U-100% 15N]	0.2-0.3 mM
2	Tris-HCl		20 mM
3	KCl		100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Release date

2008-11-10

Citation

NMR assignment of the N-terminal region of human La free and in complex with RNA
Sanfelice, D., Kelly, G., Curry, S., Conte, M.R.
Biomol. NMR Assign. (2008), 2, 107-109, PubMed 19636881 , DOI 10.1007/s12104-008-9097-5 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 15727 released on 2008-11-10
Title Full 1H, 13C, and 15N Chemical Shift Assignments for La NTD complexed with 5'UUUU RNA

Related entities 1. La NTD apo, : 1 : 7 : 37 entities Detail

Interaction partners 1. La NTD apo, : 84 interactors Detail

More details for 84 interactors identified by 29 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC