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BMRB: 15739

2K35

Hydramacin-1: Structure and antibacterial activity of a peptide from the basal metazoan Hydra

Authors

Jung, S., Dingley, A.J., Stanisak, M., Gelhaus, C., Bosch, T., Podschun, R., Leippe, M., Groetzinger, J.

Assembly

hydramacin-1

Entity

1. hydramacin-1 (polymer, Thiol state: all disulfide bound), 60 monomers, 7017.022 Da Detail

QIVDCWETWS RCTKWSQGGT GTLWKSCNDR CKELGRKRGQ CEEKPSRCPL SKKAWTCICY

Formula weight

7017.022 Da

Source organism

Hydra

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 75.8 %, Completeness (bb): 81.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.8 % (541 of 714)	80.2 % (304 of 379)	65.3 % (175 of 268)	92.5 % (62 of 67)
Backbone	81.7 % (291 of 356)	97.6 % (120 of 123)	65.1 % (114 of 175)	98.3 % (57 of 58)
Sidechain	73.8 % (305 of 413)	71.9 % (184 of 256)	78.4 % (116 of 148)	55.6 % (5 of 9)
Aromatic	14.7 % (10 of 68)	14.7 % (5 of 34)	0.0 % (0 of 29)	100.0 % (5 of 5)
Methyl	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)

1. hydramacin-1

QIVDCWETWS RCTKWSQGGT GTLWKSCNDR CKELGRKRGQ CEEKPSRCPL SKKAWTCICY

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 5.7

#	Name	Isotope labeling	Concentration
1	hydramacin-1	[U-100% 13C; U-100% 15N]	1.0 mM
2	D2O		7 %
3	H2O	natural abundance	93 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2K35, Strand ID: A Detail

Release date

2008-11-24

Citation

Hydramacin-1, structure and antibacterial activity of a protein from the basal metazoan Hydra
Jung, S., Dingley, A.J., Augustin, R., Anton-Erxleben, F., Stanisak, M., Gelhaus, C., Gutsmann, T., Hammer, M.U., Podschun, R., Bonvin, A.M., Leippe, M., Bosch, T.C., Groetzinger, J.
J. Biol. Chem. (2009), 284, 1896-1905, PubMed 19019828 , DOI 10.1074/jbc.M804713200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: B3RFR8 released on 2010-06-15
Title HYDMA_HYDVU Entity Hydramacin-1

Related entities 1. hydramacin-1, : 1 : 1 : 4 entities Detail

Experiments performed 11 experiments Detail

1 3D HNCA