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BMRB: 15740

2K36

Structure ensemble Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments, 1H-15N RDCs and 1H-1H nOe restraints for protein K7 from the Vaccinia virus

Authors

Kalverda, A.P., Thompson, G.S., Vogel, A., Schr der, M., Bowie, A.G., Khan, A.R., Homans, S.W.

Assembly

protein K7 from the Vaccinia virus

Entity

1. protein K7 from the Vaccinia virus (polymer, Thiol state: all free), 145 monomers, 16904.07 Da Detail

GAMEDAVFYF VDDDKICSRD SIIDLIDEYI TWRNHVIVFN KDITSCGRLY KELMKFDDVA IRYYGIDKIN EIVEAMSEGD HYINFTKVHD QESLFATIGI CAKITEHWGY KKISESRFQS LGNITDLMTD DNINILILFL EKKLN

Formula weight

16904.07 Da

Source organism

Vaccinia virus

Exptl. method

solution NMR

Refine. method

simulated annealing, simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 87.6 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.6 % (1527 of 1744)	85.4 % (768 of 899)	89.2 % (614 of 688)	92.4 % (145 of 157)
Backbone	93.6 % (814 of 870)	91.6 % (272 of 297)	94.9 % (406 of 428)	93.8 % (136 of 145)
Sidechain	83.3 % (843 of 1012)	82.4 % (496 of 602)	84.9 % (338 of 398)	75.0 % (9 of 12)
Aromatic	72.2 % (127 of 176)	72.7 % (64 of 88)	70.9 % (61 of 86)	100.0 % (2 of 2)
Methyl	94.3 % (164 of 174)	94.3 % (82 of 87)	94.3 % (82 of 87)

1. K7

GAMEDAVFYF VDDDKICSRD SIIDLIDEYI TWRNHVIVFN KDITSCGRLY KELMKFDDVA IRYYGIDKIN EIVEAMSEGD HYINFTKVHD QESLFATIGI CAKITEHWGY KKISESRFQS LGNITDLMTD DNINILILFL EKKLN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	K7D8	[U-13C; U-15N]	0.8 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	DSS	natural abundance	0.1 mM
6	D2O		10 %
7	H2O	natural abundance	90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
8	K7D8	[U-15N]	0.8 mM
9	sodium phosphate	natural abundance	10 mM
10	sodium chloride	natural abundance	100 mM
11	DTT	natural abundance	10 mM
12	DSS	natural abundance	0.1 mM
13	D2O		10 %
14	H2O	natural abundance	90 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
15	K7D8	[U-15N]	0.8 mM
16	sodium phosphate	natural abundance	10 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	10 mM
19	DSS	natural abundance	0.1 mM
20	Pf1 phage	natural abundance	12 mg/mL
21	D2O		10 %
22	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2K36, Strand ID: A Detail

RDC

70 RDC values in 1 lists
Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 298 K, pH 7.0 (±0.05) Detail

Release date

2008-11-10

Citation

Poxvirus K7 protein adopts a Bcl-2 fold: biochemical mapping of its interactions with human DEAD box RNA helicase DDX3
Kalverda, A.P., Thompson, G.S., Vogel, A., Schroeder, M., Bowie, A.G., Khan, A.R., Homans, S.W.
J. Mol. Biol. (2009), 385, 843-853, PubMed 18845156 , DOI 10.1016/j.jmb.2008.09.048 , Abstract

Related entities 1. protein K7 from the Vaccinia virus, : 1 : 2 : 6 entities Detail

Interaction partners 1. protein K7 from the Vaccinia virus, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC