Backbone assignment of lipid-free human apolipoprotein E (apoE)
MHHHHHHHHK VEQAVETEPE PELRQQTEWQ SGQRWELALG RFWDYLRWVQ TLSEQVQEEL LSSQVTQELR ALMDETMKEL KAYKSELEEQ LTPVAEETRA RLSKELQAAQ ARLGADMEDV CGRLVQYRGE VQAMLGQSTE ELRVRLASHL RKLRKRLLRD ADDLQKRLAV YQAGAREGAE RGLSAIRERL GPLVEQGRVR AATVGSLAGQ PLQERAQAWG ERLRARMEEM GSRTRDRLDE VKEQVAEVRA KLEEQAQQIR LQAEAAQARL KSRFEPLAED MQRQWAGQVE KVQAAEGTSA APVPSDNH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 38.1 % (1373 of 3599) | 22.5 % (428 of 1901) | 48.2 % (655 of 1359) | 85.5 % (290 of 339) |
Backbone | 67.0 % (1228 of 1832) | 51.2 % (320 of 625) | 68.2 % (619 of 907) | 96.3 % (289 of 300) |
Sidechain | 20.7 % (426 of 2058) | 8.5 % (108 of 1276) | 42.7 % (317 of 743) | 2.6 % (1 of 39) |
Aromatic | 0.0 % (0 of 164) | 0.0 % (0 of 82) | 0.0 % (0 of 76) | 0.0 % (0 of 6) |
Methyl | 23.8 % (78 of 328) | 14.6 % (24 of 164) | 32.9 % (54 of 164) |
1. Apolipoprotein E
MHHHHHHHHK VEQAVETEPE PELRQQTEWQ SGQRWELALG RFWDYLRWVQ TLSEQVQEEL LSSQVTQELR ALMDETMKEL KAYKSELEEQ LTPVAEETRA RLSKELQAAQ ARLGADMEDV CGRLVQYRGE VQAMLGQSTE ELRVRLASHL RKLRKRLLRD ADDLQKRLAV YQAGAREGAE RGLSAIRERL GPLVEQGRVR AATVGSLAGQ PLQERAQAWG ERLRARMEEM GSRTRDRLDE VKEQVAEVRA KLEEQAQQIR LQAEAAQARL KSRFEPLAED MQRQWAGQVE KVQAAEGTSA APVPSDNHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Apolipoprotein E | [U-100% 13C; U-100% 15N; 80% 2H] | 1 mM | |
2 | phosphate buffer | 25 mM | ||
3 | NaCl | 25 mM | ||
4 | NaN3 | 0.05 mM | ||
5 | D2O | 5 % | ||
6 | DTT-d10 | 10 mM | ||
7 | EDTA | 10 mM |